Le informazioni nella sezione "Riassunto" possono far riferimento a edizioni diverse di questo titolo.
1. Assessing b-amyloid-induced NLRP3 Inflammasome Activation in Primary Microglia
Mareike Schnaars, Hannes Beckert, and Annett Halle
2. Activating the NLRP3 Inflammasome using the Amyloidogenic Peptide IAPP
Clara Westwell-Roper, Aisling Dunne, Man-Lyang Kim, C. Bruce Verchere, and Seth L. Masters
3. Assessment and Quantification of Crystal-induced Lysosomal Damage
Peter Duewell and Eicke Latz
4. Assessment of Inflammasome Activation in Primary Human Immune Cells
Theo S. Plantinga, Leo A.B. Joosten, and Mihai G. Netea
5. NLRP3 Inflammasome Activation and Cytotoxicity Induced by particulate Adjuvants
Marie Yang, Claire H.A. Hearnden, Ewa Oleszycka and Ed C. Lavelle
6. Measuring Inflammasome Activation in Response to Bacterial Infection
Petr Broz and Denise M. Monack
7. Detection of Pyroptosis by Measuring Released Lactate Dehydrogenase Activity
Manira Rayamajhi, Yue Zhang, and Edward A. Miao
8. ASC Speck Formation as a Read-out for Inflammasome Activation
Andrea Stutz, Gabor L. Horvath, Brian G. Monks, and Eicke Latz
9. Immunoblotting for Active Caspase-1
Christopher Jakobs, Eva Bartok, Andrej Kubarenko, Franz Bauernfeind and Veit Hornung
10. Inflammasome Activation and Inhibition in Primary Murine Bone Marrow-Derived Cells, and Assays for IL-1α, IL-1β and Caspase-1
Katharina S. Schneider1, Christina J. Thomas, and Olaf Groß
11. Reconstituting the NLRP1 Inflammasome in vitro
Benjamin Faustin and John C. Reed
12. Assessing ATP Binding and Hydrolysis by NLR Proteins
Jinyao Mo and Joseph A. Duncan
13. Blue Native Polyacrylamide Gel Electrophoresis to Monitor Inflammasome Assembly and Composition
Eric M. Kofoed and Russell E. Vance
14. Analysis of Microbiota Alterations in Inflammasome-deficient Mice
Eran Elinav, Christoph A Thaiss, and Richard A Flavell
15. Quantification of Adipose Tissue Leukocytosis in Obesity
Ryan Grant, Yun-Hee Youm, Anthony Ravussin, and Vishwa Deep Dixit
16. In vivo Evaluation of Neutrophil Recruitment in Response to Sterile Particulates
Tamiko Yanagida, Kaoru Orihashi and Hajime Kono
Inflammasomes have a seemingly simple architecture. A sensor molecule of the NLR or PYHIN protein family recruits the common adapter molecule apoptosis-associated speck-like protein containing a CARD (ASC). Even so, inflammasome activation is highly regulated at multiple steps and precise details of how inflammasomes are activated at the molecular level remain largely unknown. In The Inflammasome: Methods and Protocols, expert researchers in the field detail methods and protocols to study multiple aspects of inflammasome function. These methods include the generation of inflammasome stimuli, perturbance of cellular homeostasis, assessment of ASC speck formation, monitoring of caspase-1 activity and processing, activation of IL-1ß cytokines, and analysis of inflammasome assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Le informazioni nella sezione "Su questo libro" possono far riferimento a edizioni diverse di questo titolo.
Spese di spedizione:
GRATIS
In U.S.A.
Descrizione libro Hardcover. Condizione: new. Codice articolo 9781627035224
Descrizione libro Condizione: New. Codice articolo ABLIING23Mar2811580170137
Descrizione libro Gebunden. Condizione: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Presents current up-to-date methodologiesProvides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the expertsThis Methods in Molecular Biology book offers methods for studying. Codice articolo 4267649
Descrizione libro Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This Methods in Molecular Biology book offers methods for studying inflammasome function, including generation of inflammasome stimuli, monitoring of caspase-1 activity and processing, activation of IL-1beta cytokines, plus lab protocols, material lists and tips. 240 pp. Englisch. Codice articolo 9781627035224
Descrizione libro Buch. Condizione: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - This Methods in Molecular Biology book offers methods for studying inflammasome function, including generation of inflammasome stimuli, monitoring of caspase-1 activity and processing, activation of IL-1beta cytokines, plus lab protocols, material lists and tips. Codice articolo 9781627035224