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Part One: Basic Microbiology
Introduction to Microbiology
Exercise: 1 Ubiquity of Microorganisms
Exercise: 2 Bright-Field Light Microscopy, Including History and Working Principles
Exercise: 3 Microscopic (Bright-Field and Dark-Field) Determination of Cell Motility, Form, and Viability Using Wet Mount and Hanging Drop Preparations
Introduction to Staining of Microorganisms
Exercise: 4 Simple Stains: Positive and Negative Stains
Exercise: 5 Differential and Special Stains Introduction to Microbial Growth
Exercise: 6 Pure Culture and Aseptic Technique
Exercise: 7 Chemically Defined, Complex, Selective, and Differential Media
Exercise: 8 Quantification of Microorganisms
Introduction to the Environment and Microbial Growth
Exercise: 9 Aerobic and Anaerobic Growth
Exercise: 10 The Effect of Incubation Temperature on Generation Time
Introduction to Control of Microbial Growth
Exercise: 11 Moist and Dry Heat Sterilization: Thermal Death Point and Thermal Death Time
Exercise: 12 Control of Microbial Growth with Ultraviolet Light
Exercise: 13 Osmotic Pressure, and Its Effect on Rate and Amount of Microbial Growth
Exercise: 14 Antiseptics and Antibiotics
Introduction to Microbial Genetics
Exercise: 15 Selection of Bacterial Mutants Resistant to Antibiotics
Exercise: 16 Transformation: A Form of Genetic Recombination
Exercise: 17 Bacterial Conjugation
Exercise: 18 Gene Regulation: Induction and Catabolite Repression
Part Two: The Other Microbial World
Introduction to the Other Microbial World
Exercise: 19 Microscopic Identification of Fungi
Exercise: 20 Parasitology: Protozoa and Helminths
Exercise: 21 Titering Prokaryotic Viruses
Part Three: Microbes and Humans
Introduction to Medical Microbiology
Exercise: 22 Normal Skin Flora
Exercise: 23 Respiratory Microorganisms
Exercise: 24 Identification of Enteric Gram-Negative Rods
Exercise: 25 Clinical Unknown Identification
Part Four: Immunology
Introduction to Some Immunological Principles and Techniques
Exercise: 26 Differential White Blood Cell Stains
Exercise: 27 Lysozyme, an Enzymatic Form of Natural Resistance
Exercise: 28 Lancefield Grouping of Pathogenic Streptococci with a Latex Slide Agglutination Test
Exercise: 29 Use of an Enzyme-Linked Immunosorbent Assay (ELISA) Test for Coccidioides Immitis Identification
Exercise: 30 An Ouchterlony Double Immunodiffusion Test for Coccidioides Immitis Identification
Part Five: Public Health
Introduction to the Prevention and Control of Communicable Diseases
Exercise: 31 Epidemiology: A Staphylococcus Carrier Study
Exercise: 32 Bacteriological Examination of Water: Multiple-Tube Fermentation and Membrane Filter Techniques
Part Six: Applications of Biotechnology
Introduction to Biotechnology
Exercise: 33 Identifying DNA with Restriction Enzymes
Exercise: 34 Identification of Bacteria Using the Ribosomal Data Project
Part Seven: Projects
Introduction to the Individual Projects
Exercise: 35 Hydrocarbon-Degrading Bacteria, Cleaning Up After Oil Spills
Exercise: 36 Luminescent Bacteria: Bacteria That Produce Light
Exercise: 37 Methylotrophs, Organisms That Grow on One-Carbon Compounds
Exercise: 38 Deinococcus, Bacteria with Out-Of-This-World Capabilities
Appendices
1. Living Microorganisms (Bacteria, Fungi, Protozoa, and Helminths) Chosen for Study in this Manual
2. Dilution Practice Problems
3. Metric System, Use of with Conversions to the English System of Measurement
4. Alternative Procedures
5. Use of the Ocular Micrometer for Measurement of Relative and Absolute Cell Size
6. Use of the Hemocytometer for Determining Total Cell Number in a Liquid Suspension
7. Preparation of Covered Slide Cultures for Study of Intact Structure of a Mold Thallus
8. Additional Reading
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