Manipulation and Expression of Recombinant DNA: A Classroom Laboratory Manual

Carson, Sue; Robertson, Dominique

ISBN 10: 0120884186 ISBN 13: 9780120884186

Casa editrice: Academic Press, 2005

Riassunto

This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein.

The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein―students can actually visualize positive clones following IPTG induction.

*Cover basic concepts and techniques used in molecular biology research labs
*Student-tested labs proven successful in a real classroom laboratories
*Exercises simulate a cloning project that would be performed in a real research lab
*"Project" approach to experiments gives students an overview of the entire process
*Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions

Su questo libro

Product Description:

Book by Carson Sue Robertson Dominique

Su questa edizione

• Editore: Academic Press
• Data di pubblicazione: 2005
• ISBN 10: 0120884186
• ISBN 13: 9780120884186
• Rilegatura: Pettine di plastica
• Numero edizione: 2
• Numero di pagine: 176