Experimental Neuroanatomy: A Practical Approach (Practical Approach Series): 114 - Brossura

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ISBN 10: 0199633258 ISBN 13: 9780199633258

Casa editrice: Oxford University Press, USA, 1993

Riassunto

A dramatic increase in the number of practical techniques available to the experimental neuroanatomist over the past decade has led to major advances in the knowledge and understanding of the nervous system. This book is an essential laboratory manual for experimental neuroscientists, with an emphasis on ultrastructural analysis. It contains detailed protocols for the preparation of specimens, perfusion-fixation, and the preparation of sections for light and electron microscopy. It covers up-to-date neuroanatomical techniques including procedures for tract-tracing and chemical characterization of neurons and their processes. These procedures, either alone or in combination, are being widely used to elucidate complex neuronal interactions. Experimental Neuroanatomy" A Practical Approach is an invaluable text for neuroanatomists and other neuroscientists who wish to apply these fascinating techniques.

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Recensione:

'an essential handbook for the neuroanatomist ... The book is well illustrated, readable and comprehensive ... this handbook would make a very good introductory read for the beginner and is ideal for the experienced investigator who wishes to add to her/his repertoire or to brush up on existing techniques. As with all books of the series, it is a good buy and easily affordable.'Z. Henderson, Neuroscience, Vol. 58, No. 2, 1994

'an essential handbook for the neuroanatomist ... The book is well illustrated, readable and comprehensive and it covers all the techniques available today for the study of the microcircuitry of the adult brain ... this handbook would make a very good introductory read for the beginner and is ideal for the experienced investigator who wishes to add to her/his repertoire or to brush up on existing techniques. As with all books of the series, it is a good buy and easily affordable.'Z. Henderson, Neuroscience, Vol. 58, No. 2, 1994

Contenuti:

J.P. Bolam: Preparation of central nervous system tissue for light and electron microscopy; I.J. Llewellyn-Smith, P. Pilowsky, & J.B. Minson: Retrograde tracers for light and electron microscopy; Y. Smith: Anterograde tracing with PHA-L and biocytin at the electron microscopic level; P.L. Strick & J.P. Card: Transneuronal mapping of neural circuits with alpha herpesviruses; S. Totterdell, C.A. Ingham, & J.P. Bolam: Immunocytochemistry I: pre-embedding staining; C.A. Ingham: Immunocytochemistry II: post-embedding staining; S.R. Vincent: Histochemistry of endogenous enzymes; C.R. Gerfen, W.S. Young, III, & P. Emson: In situ hybridization histochemistry with oligonucleotides for localization of messenger RNA in neurones; E.H. Buhl: Intracellular Lucifer Yellow injection in fixed brain slices; D.J. Maxwell: Intracellular staining in vivo; Y. Smith, & J.P. Bolam: Combined approaches to experimental; neuroanatomy: combined tracing and immunocytochemical techniques for the study of neuronal microcircuits; Appendix; Index.

Su questa edizione

• Editore: Oxford University Press, USA
• Data di pubblicazione: 1993
• ISBN 10: 0199633258
• ISBN 13: 9780199633258
• Rilegatura: Copertina flessibile
• Numero di pagine: 296
• Redattore: Bolam J. P.