Gene Probes 2: A Practical Approach - Brossura

Brossura

ISBN 10: 0199634149 ISBN 13: 9780199634149

Casa editrice: IRL Press, 1995

Riassunto

Gene probes find a wide variety of uses in molecular biology, and are essential tools for genetic research. This volume describes some of their many applications, from the study of nucleic acids, through blotting techniques and gene targeting, to analysis of human genetic mutations. A Companion volume, Genes Probes 1, covers preparation of the probes. The two books are timely additions to the series, as this is a progressive field which continues to grow in importance.

Su questo libro

L'autore

S. J. Higgins is at University of Leeds.

Contenuti

• 1.: Hybridization strategy
• 2.: Screening recombinant DNA libraries by hybridization and amplification
• 3: Screening human recombinant libraries with nucleic acid probes
• 4.: Antibody screening of bacterial expression systems
• 5.: Isolation of cDNA clones by direct expression in mammalian cells
• 6.: Genomic mapping and gene structure analysis
• 7.: Analysis of gene expression by blotting techniques
• 8.: In situ hybridization of nucleic acid probes to cellular RNA
• 9.: Applications of chromosomal in situ hybridization
• 10.: Analysis of gene expression analysis by PCR: a quantitative approach
• 11.: Analysis of protein expression by immunocytochemistry
• 12.: Gene targeting in mammalian cells
• 13.: Directed mutagenesis and mutant analysis
• 14.: Human gene mutational analysis
• Appendix 1
• Appendix 2

Dettagli dell?edizione

• Editore: IRL Press
• Data di pubblicazione: 1995
• ISBN 10: 0199634149
• ISBN 13: 9780199634149
• Rilegatura: Copertina flessibile
• Lingua: Inglese
• Numero di pagine: 446
• Redattore: Hames B. D., Higgins S. J.
• Contatto del produttore: non disponibile