Real-time PCR - Brossura

Brossura

ISBN 10: 041537734X ISBN 13: 9780415377348

Casa editrice: Routlegde, 2006

Riassunto

With a variety of detection chemistries, an increasing number of platforms, multiple choices for analytical methods and the jargon emerging along with these developments, real-time PCR is facing the risk of becoming an intimidating method, especially for beginners. Real-time PCR provides the basics, explains how they are exploited to run a real-time PCR assay, how the assays are run and where these assays are informative in real life. It addresses the most practical aspects of the techniques with the emphasis on 'how to do it in the laboratory'. Keeping with the spirit of the Advanced Methods Series, most chapters provide an experimental protocol as an example of a specific assay.

Su questo libro

L'autore:

Tevfik Dorak-Medical Department, University of Newcastle upon Tyne, Newcastle, UK

Contenuti:

1. An Introduction to Real-time PCR  2. Data Analysis and Reporting  3. Relative Quantification  4. Normalisation  5. High-Throughput Primer and Probe Design  6. Quantitative Analysis of Ocular Gene Expression  7. Quantitative Gene Expression by Real-time PCR  8. Real-time PCR Using SYBR Green  9. High-Resolution Melting Analysis for Scanning and Genotyping  10. Quantitative Analyses of DNA Methylation  11. Mitochondrial DNA Analysis  12. Real-time Immuno-PCR  13. Clinical Microbiology  14. Clinical Virology  15. Solid Organ Transplant Monitoring  16. Real-time PCR Applications in Haematology  17. Real-time PCR for Prenatal Diagnosis of Monogenic Diseases Caused by Single Nucleotide Changes - the Example of the Haemoglobinopathies

Su questa edizione

• Editore: Routlegde
• Data di pubblicazione: 2006
• ISBN 10: 041537734X
• ISBN 13: 9780415377348
• Rilegatura: Copertina flessibile
• Numero edizione: 1
• Numero di pagine: 362
• Redattore: Dorak M