This book describes techniques used in the study of transcription factors and DNA sequences involved in regulating gene transcription. The various methods used to analyze protein—DNA interactions such as electrophoretic mobility shift assay are described together with other more sophisticated footprinting techniques. Practical guidance is also provided on cloning and characterizing transcription factors and analyzing regulatory sequences, together with a description of database searching by homology. The major techniques employed in studying transcriptional regulation such as transcript analyses, transfection of DNA into cells and the use of reporter constructs are described in the companion volume to this book Gene Transcription: RNA Analysis. The Essential Techniques Series books are designed to provide you with immediate access to the protocols you require every day. These handy pocket-sized manuals are easy to carry around, and conveniently spiral bound making them ideal for lab bench work. Written by experienced laboratory researchers, each book in the Essential Techniques Series gives up-to-date, tried and tested practical information for the life scientist. For each key technique these books:
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This essential laboratory reference describes the techniques used in the study of transcription factors and DNA sequences that are involved in regulating gene transcription. The various methods used to analyze protein-DNA interaction, such as electrophoretic mobility shift assay are described along with more sophisticated footprinting techniques. The editor also provides guidance on cloning and characterizing transcription factors, analyzing regulatory sequences, and the best choice of method, conditions, equipment and reagents.
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Descrizione libro John Wiley & Sons, 1997. Spiral-bound. Condizione libro: Used: Good. Codice libro della libreria SONG0471970166