Gene Cloning and Manipulation - Rilegato

Howe, Christopher

Rilegato

ISBN 10: 0521403413 ISBN 13: 9780521403412

Casa editrice: Cambridge University Press, 1995

Riassunto

This text gives a broad, but concise, coverage of gene cloning and manipulation, suitable for undergraduates and beginning graduate students. Assuming only general biochemical knowledge, it stresses the concepts underlying particular types of cloning vector, and uses examples to illustrate them, rather than simply presenting a mass of detailed lists and vector maps. The book starts by describing the principles behind cloning DNA in E. coli, the enzymes used, the range of cloning vectors available, and how to screen libraries to find particular clones. The author shows how PCR can be used as an alternative, or complementary, approach. He then goes on to describe how sequences can be exploited, after cloning and identification, by site-directed mutagenesis and over-expression. The book finishes with a detailed presentation of the genetic manipulation of other organisms, including other bacteria, yeast, plants, insects, and mammals.

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Descrizione del libro

Genetic engineering underlies most modern biological research. This book explains the methods, stressing the underlying principles, to undergraduates and new researchers. It covers the genetic manipulation of animals and plants as well as micro-organisms and combines broad coverage with a concise, but thorough, treatment.

Contenuti

Part I. The Tools for the Job: 1. Introduction; 2. Cutting; 3. Modification; 4. Ligation; 5. Purification of plasmid DNA from E. Coli; 6. Gel electrophoresis of nucleic acids; 7. Oligonucleotide synthesis; Part II. Simple Cloning: 8. The basic experiment; 9. Vectors; transformation and hosts; 10. Modifications; 11. Linkers, adaptors and cassettes; Part III. Other vector systems for E. Coli; 12. Introduction; 13. Bacteriophage M13 and its uses; 14. Bacteriophage lambda; 15. Cosmids; 16. Bacteriophage P1; Part IV. Making Libraries: 17. Introduction; 18. Genomic libraries; 19. cDNA libraries; 20. Specialized libraries; Part V. Screening Libraries: 21. Introduction; 22. Screening for coding function; 23. Screening for other functions-reporter genes; Part VI. Polymerase Chain Reaction: 24. The basic technique; 25. Modifications; 26. Precautions and drawbacks; 27. Modification and mutagenesis; 28. Introduction; 29. Alteration of restriction sites; 30. Insertions and deletions; 31. Point mutations - early methods; 32. Oligonucleotide mutagenesis; 33. Choosing the right mutations; 34. Inactivating genes; Part VII. Use of Cloned DNA: 35. As DNA; 36. Synthesis of RNA; 37. Synthesis of protein; Part VIII. Using Other Organisms: 38. Gram-negative bacteria; 39. Gram-positive bacteria; 40. Fungi; 41. Chlamydomonas; 42. Vascular plants; 43. Organelle transformation; 44. Insects; 45. Mammals; References and further reading; Index.

Dettagli dell?edizione

• Editore: Cambridge University Press
• Data di pubblicazione: 1995
• ISBN 10: 0521403413
• ISBN 13: 9780521403412
• Rilegatura: Copertina rigida
• Lingua: Inglese
• Numero di pagine: 224