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Sinossi
Updated to reflect advances in the field, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of 'real-life' biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams.
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Descrizione del libro
Updated to reflect advances in the field, this introduction provides a broad but concise coverage of recombinant DNA techniques. It is written specifically for students and researchers who want to use this technology, with emphasis placed on understanding the underlying concepts, thereby enabling the reader to devise their own strategies.
L'autore
Christopher Howe is Professor of Plant and Microbial Biochemistry at the University of Cambridge. His research interests include biochemistry and molecular biology of photosynthetic organisms, and the evolutionary processes that gave rise to them. He has taught molecular biology for twenty years.
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- EditoreCambridge University Press
- Data di pubblicazione2007
- ISBN 10 052152105X
- ISBN 13 9780521521055
- RilegaturaCopertina flessibile
- LinguaInglese
- Numero edizione2
- Numero di pagine278
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Gene Cloning and Manipulation
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Rustica (tapa blanda). Condizione: New. Condizione sovraccoperta: Nuevo. 1. Updated to reflect advances in the field, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed on the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. LIBRO. Codice articolo 172933
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Condizione: Gut. Herausgeber : Cambridge University Pr., 2. Edition (12. Juli 2007) Sprache : Englisch Taschenbuch : 278 Seiten ISBN-10 : 052152105X ISBN-13 : 978-0521521055 Now fully updated to reflect recent advances, this introduction provides a broad, but concise, coverage of recombinant DNA techniques. Written for advanced undergraduates, graduates and scientists who want to use this technology, emphasis is placed an the concepts underlying particular types of cloning vectors to aid understanding and to enable readers to devise suitable strategies for novel experimental situations. An introduction to the basic biochemical principles is presented first. Then PCR and cloning using E. coli hosts and plasmid, phage and hybrid vectors are described, followed by the generation and screening of libraries and how to modify, inactivate or express cloned sequences. Finally, genetic manipulation in a range of other organisms is discussed, including other bacteria, fungi, algae and plants, insects and mammals. A series of , real-life, biological problems are also presented to enable readers to assess their understanding of the material and to prepare for exams. is Professor of Plant and microbial Biochemistry at the University of Cambridge. His research interests include biochemistry and molecular biology of photosynthetic organisms, and the evolutionary processes that gave rise to them. He has taught molecular biology for 20 years. gutes Exemplar, ordentlich, Gern können sie Ihr Buch per Rechnung bestellen. Hardcover. Codice articolo B00070420
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