High Performance Liquid Chromatography: Fundamental Principles and Practice

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9780751400762: High Performance Liquid Chromatography: Fundamental Principles and Practice

High performance liquid chromatography (HPLC) has long been recognized as one of the most useful and versatile analytical techniques. It has now progressed from being a highly expensive method of analysis to a routine technique with wide applications. Consequently there is a requirement in many chemistry and chemistry-related courses for students to acquire a detailed understanding of the principles and practice of HPLC.

Written in a manner suitable for undergraduate students studying analytical chemistry and learning about chromatographic analytical techniques applied to pharmaceutical analysis, biochemistry and related disciplines, High-performance Liquid Chromatography: Fundamental Principles and Practice introduces the fundamentals of HPLC. Loosely structured in three parts, the text begins with a thorough introduction of the subject and then progresses through the essential knowledge of the instrumentation needed for HPLC. The final part covers with the applications of HPLC in real-world situations. Developed by a team of international experts from a wide cross-section of disciplines, the text is relevant to a wide range of courses.

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Contenuti:

INTRODUCTION, W.J. Lough and I.W. Wainer
Analysis and Chromatography
Historical Development of Chromatography
HPLC Today

EFFICIENCY, RETENTION, SELECTIVITY, AND RESOLUTION IN CHROMATOGRAPHY, C.M. Riley
Introduction
Chormatographic Mobility
Peak Shape
Retention Relationships
Band Broadening and Column Efficiency
Separation

MODES OF CHROMATOGRAPHY, C.M. Riley
Terminology
Chromatographic Format
Models of Retention
Choice of Mode of Chromatography
Normal-Phase Liquid Chromatography
Reversed-Phased Liquid Chromatography
Ion-Exchange Chromatography
Size-Exclusion Chromatography

SUPPORT MATERIALS AND SOLVENTS, P. Hambleton
Adsorption Chromatography
Chemically Bonded Silica
Polymer Packings
Porous Graphitic Carbon
Solvents for HPLC

INSTRUMENTATION: PUMPS, INJECTORS, AND COLUMN DESIGN, T. Noctor
Introduction
Types of HPLC Pump
Approaches to the Reduction of Flow Pulsation
The Modern HPLC Pump
Valve Injectors
Column Dimemsions
Column Inlet/Outlet
Connecting Tubing and Unions
Conclusion

INSTRUMENTATION: DETECTORS AND INTEGRATORS, D.K. Lloyd
Ideal Detectors and Real Detectors
Detector Performance Criteria
The UV-Visible Absorbance Detector
The Fluorescence Detector
The Refractive Index Detector
Electrochemical Detectors
Other Detection Methods
Integrators

METHOD DEVELOPMENT AND QUANTITATION, W.J. Lough and I.W. Wainer
Method Development
Optimisation
Relative Assays
Method Validation
Types of Quantitative Method

SAMPLE PREPARATION, D. Stevenson
Introduction
Sample Preparation Procedures
Matrix Properties
Analyte Propeties
Examples of Applications
Automation
Concluding Remarks

POLYMER ANALYSIS, A.J. Handley
Introduction
Chromatography Modes Used for Polymer Analysis
Size Exclusion Chromatography
Other Modes of Chromatography

HPLC IN BIOMEDICAL AND FORENSIC ANALYSIS, D. Perrett and P. White
Introduction
Biomedical Analysis
Forensic Analysis

ENVIRONMENTAL ANALYSIS, P.J. Renne
Introduction
HPLC Mode
Sample Preparation
Choice of Column
Pumping System
Detectors
Sample Introduction
Analytes
Miscellaneous
Suggested Protocol
Conclusions

FOOD, ORGANIC, AND PHARMACEUTICAL APPLICATIONS, W.J. Lough and I.W. Wainer
Introduction
Bulk Substances
Products or 'Formulations'
Conclusion

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Editore: CRC Press (1995)
ISBN 10: 0751400769 ISBN 13: 9780751400762
Nuovi Paperback Quantità: 1
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Descrizione libro CRC Press, 1995. Paperback. Condizione libro: New. book. Codice libro della libreria 0751400769

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