PCR Primer: A Laboratory Manual - Brossura

Brossura

ISBN 10: 0879694483 ISBN 13: 9780879694487

Casa editrice: Cold Spring Harbor Laboratory Press,U.S., 1995

Riassunto

The polymerase chain reaction has become a standard research tool in a range of laboratories. Its impact has been felt in basic molecular biological research, clinical research, forensics, evolutionary studies, and the Human Genome Project. The PCR technique originally conceived by Nobel laureate Kary Mullis has proven to be exceptionally adaptable and has been transformed into a myriad array of methods, each with different applications. This manual introduces the complex world of PCR by beginning at an accessible level and then moving to more advanced levels of application. First, the practical requirements for performing PCR and other amplification techniques in the lab are introduced and then the basic aspects of the technique are explained by exploring important issues such as sample preparation, primer design, efficiency, detection of products, and quantitation. Protocols for a range of PCR and amplification techniques are presented for cloning, sequencing, mutagenesis, library construction and screening, exon trapping, differential display, and expression, and these include RT-PCR, RNA PCR, LCR, multiplex PCR, panhandle PCR, capture PCR, expression PCR, 3' and 5' RACE, in situ PCR, and litigation-mediated PCR. Each protocol is augmented by analysis and troubleshooting sections and complete references.

Su questa edizione

• Editore: Cold Spring Harbor Laboratory Press,U.S.
• Data di pubblicazione: 1995
• ISBN 10: 0879694483
• ISBN 13: 9780879694487
• Rilegatura: Copertina flessibile
• Numero di pagine: 714
• Redattore: Dieffenbach Carl W., Dveksler Gabriela S.