D ring the past ten ears or so, there has been an increasing n mber of p blications describing ario s aspects of oligon cleotide chemistr and the application of oligon cleotides in basic, as ell as applied science. More recentl , ario s oligon cleotide conj gates nthesi ed that are c rrentl being st died for their ha e been s potential diagnostic and therape tic applications. With the introd- tion of ne oligon cleotide conj gates, anal tical methods for the anal sis of these oligon cleotides are also being de eloped and op- mi ed. The present ol me, Protocols for Oligon cleotide Con- gates: S nthesis and Anal tical Techniq es, is designed to bring readers p-to-date on the protocols for the s nthesis of ario s oli- gates and on all the important techniq es for the n cleotide conj nthetic oligon cleotides and their analogs. The s- anal sis of s thetic chemistrof man of these oligon cleotides and their ario s analogs has been co ered in a companion ol me entitledProtocols for Oligon cleotides and Analogs: S nthesis and Properties. The first chapter ofProtocols for Oligon cleotide Conj gates- scribes the protecting gro ps in oligon cleotide s nthesis.
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...an extremely useful and well-produced volume...a very well-qualified and prestigious group of authors...produce an up-to-date review of current topics in this important and growing field. - Trends in Biotechnology
Protecting Groups in Oligonucleotide Synthesis Incorporation of Modified Bases into Oligonucleotides Functionalization of Oligonucleotides with Amino Groups and Attachment of Amino Specific Reporter Groups Functionalization of Oligonucleotides by the Incorporation of Thio-Specific Reporter Groups Postsynthesis Functionalization of Oligonucleotides Oligonucleotide-Enzyme Conjugates Oligonucleotides Containing Degenerate Bases: Synthesis and Uses Synthesis of [15N]-Labeled DNA Fragments Analysis and Purification of Synthetic Oligonucleotides by High Performance Liquid Chromatography Sequence Analysis of Oligodeoxyribonucleotides Gel-Capillary Electrophoresis Analysis of Oligonucleotides Nuclear Magnetic Resonance Studies of Oligonucleotides Mass Spectrometry of Nucleotides and Oligonucleotides Extracting Thermodynamic Data from Equilibrium Melting Curves for Oligonucleotide Order-Disclosure Transitions Index
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