This new edition takes account of the progress that has been made in the field since the publication of the first edition in 1986. Although the basic principles remain the same, significant advances have been made in areas such as serum-free media, scale-up, and flow cytometry. As these techniques have developed as tools for the cell biologist, their availability to the non-specialist has also increased dramatically. Use of the tetrazolium salt MTT as a colorimetric indicator of viability has made a considerable impact on cytotoxicity assay, and DNA fingerprinting has revolutionized the identification of individual cell strains. These, and other developments in the techniques described have made this new edition essential. The emphasis remains on presenting techniques in a readily accessible form, with detailed protocols given throughout. This volume should be of use to researchers involved in both biological research and the commercial exploitation of animal cell culture.
R. I. Freshney, CRC Department of Medical Oncology, University of Glasgow.Review:
'this book contains plenty of theoretical background, making it a useful text for undergraduates following courses in cell biology or biotechnology ... I would strongly recommend this book, both as a general and as a specific guide to the subject.'
Maureen Dawson, Manchester Metropolitan University, Tibtech, October 1993 (Vol 11)
'Most researchers using cell cultures will find this book contains valuable information that will save them time and money.'
International Journal of Biochemistry, Vol. 25, No. 9, 1993
'a useful introduction to tissue culture for students and trainee technicians'
Ian Freshney, BioEssays, Vol. 16, No. 3 - March 1994
Descrizione libro Oxford University Press, 1986. Paperback. Condizione libro: New. book. Codice libro della libreria 947946330