Content.- Developments and Challenge of Enzyme Engineering.- 1973 Henniker Delphi Study.- Session I. Enzymes: Regulation and New Sources.- Stability of Enzymes from Thermophilic Microorganisms.- Polypeptide Hormones from Tissue Culture.- Design Consideration for Animal Cell Cultures as Sources of Enzymes.- Session II. New Purification Techniques.- Recent Developments in Enzyme Isolation Processes.- Protein Purification by Immunoadsorption.- Purification of Urokinase by Affinity Chromatography.- Short Report:.- Derivatives of Controlled Pore Glass for Affinity Chromatography.- Session III. New Immobilization Techniques and Supports.- Review of Recent Enzyme Immobilization Techniques.- Encapsulation of Enzymes in Liquid Membrane Emulsions.- An Organic Millieu in Immobilized Enzyme Synthesis and Catalysis.- Immobilization of Enzymes on Phenol-Formaldehyde Resins.- Derivatized Nylon: A New Support for the Immobilization of Enzymes.- New Immobilization Techniques and Supports.- Stabilization and Immobilization of Enzymes with Imidoesters.- Complexation of Enzymes or Whole Cells with Collagen.- Short Reports:.- Immobilized Enzymes Using Resinous Carriers.- Covalent Coupling of Small Molecules and Proteins to Poly (2-Hydroxyethyl Methacrylate Methacrylic Acid) Hydrogels.- Session IV. Immobilized Multi-Step Enzyme Systems: Physical Methods for Examining Immobilized Enzymes.- Theoretical and Practical Aspects of Immobilized Multi-Step Enzyme Systems.- Enzyme Reactions for Preparative Scale Synthesis.- Application of Circular Dichroism to the Study of Enzyme Polymer Conjugates.- Fluorescence Investigation of Immobilized Enzymes.- Effects of the Matrix Environment on Protein Structure.- Short Reports:.- Electron Spin Resonance Investigations into the Interaction Between Proteins and Synthetic Polymers.- Some Observations on the Behavior of an Immobilized Allosteric Enzyme: Phosphofructokinase.- Session V. Use of Immobilized Coenzymes.- Nucleotide Affinity Labels.- Continuous Processing with Cofactor Requiring Enzymes: Coenzyme Retention and Regeneration.- NADH Coupled to Soluble Dextran: Synthesis and Properties.- Enzymatic Regeneration of ATP from AMP and ADP Part I. Thermodynamics, Kinetics and Process Development.- Enzymatic Regeneration of ATP from AMP and ADP Part II. Enzyme Immobilization and Reactor Development.- ATP Regeneration Using Immobilized Carbamyl Phosphokinase.- Immobilization of Coenzyme A and Its Application.- Synthesis and Application of Matrix Bound AMP, NAD+ and Other Adenine Nucleotides.- Session VI. Reactor Design.- Well-Mixed Immobilized Enzyme Reactors.- Reactor Analysis with Laser Microspectrophotometry.- Open Tubular Heterogeneous Enzyme Reactors.- Annular Column Enzyme Reactors.- Kinetic Analysis of a Urease Electrode.- Short Reports:.- Temperature Effects on the Design of Enzyme Reactors.- Immobilized Enzymes: Diffusion and Sigmoid Kinetics in Packed Bed Reactors.- Session VII. Industrial Applications of Immobilized Enzymes.- Food and Product Considerations in the Application of Immobilized Enzymes.- Fiber Entrapped Enzymes.- Continuous Enzyme Reactions by Immobilized Microbial Cells.- Immobilized Glucose Isomerase for the Production of High Fructose Syrups.- Treatment of Milk with Immobilized Proteases and Oxidoreductases.- Short Reports:.- Removal of Serum Hepatitis Antigen from Factor IX with an Immunoadsorbent.- Immobilized Lactase Used in Fluidized Bed Reactors for Treating Cheese Whey.- Session VIII. Commercial Aspects of Enzymes.- Application of a Heat Stable Bacterial Amylase in the Starch Industry.- Toxicologic Studies on Proteolytic Enzymes in Animals and Man.- Recent Trends of Enzyme Engineering in Japan.- Survey of the Enzyme Industry.- Session IX. New Applications of Enzymes.- Analytical Uses of Immobilized Enzymes.- Application of Immobilized Enzymes to Chemical Analysis.- Application of Immobilized Enzymes to Detection and Monitoring.- Preparation of Immobilized Enzymes for Application in Auto
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