In recent years, molecular imaging techniques have grown to be invaluable tools for molecular biology research and, to a more modest extent, clinical medicine. In Molecular Imaging: Methods and Protocols, expert researchers explore the latest advances in the field, describing a rich variety of practical procedures and methods for diverging imaging technologies. Chapters are divided into sections that cover the imaging of basic molecular and biochemical events, imaging in pre-clinical settings, and imaging in clinical settings. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.Comprehensive and ground-breaking, Molecular Imaging: Methods and Protocols is an essential handbook for students, established practitioners, and research fellows alike.
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From the reviews:
“This handbook describes a rich variety of practical procedures and methods for diverging imaging technologies. ... This sufficiently detailed handbook is essential for students, research fellows, and established practitioners from different fields to become familiar with molecular imaging and incorporate it into their work. I highly recommend the book to trainees and practitioners in nuclear medicine and radiology with research interest in molecular imaging.” (E. Edmund Kim, The Journal of Nuclear Medicine, Vol. 53 (1), January, 2012)
“The book covers many aspects of molecular imaging at the cellular and subcellular level and within intact living organisms. The techniques are well described and protocols are provided in detail, with a lot of technical specifications, also concerning instrumentation settings and the software used. All this information is of fundamental importance for imaging measurements and is seldom reported in the scientific literature. .... can be recommended to students, research fellows, and technicians working or planning to work with molecular imaging techniques.” (Elisa Michelini, Analytical and Bioanalytical Chemistry, Vol. 403, 2012)
“Excellently presented, this book on molecular imaging provides valuable information on practical procedures and methods for divergent technologies for imaging of basic molecular and biochemical events in addition to imaging in preclinical and clinical settings. ... The book is intended for students, established practitioners, and research fellows from other fields who want to become familiar with molecular imaging ... . graduate students, practicing radiologists and imaging experts, research fellows, and anyone interested in molecular imaging will find this book valuable.” (Omer Iqbal, Doody's Book Reviews, March, 2011)Molecular Imaging: Methods and Protocols Editor Khalid Shah, PhD TABLE OF CONTENTS Table of Contents Preface Contributors Imaging Biochemical Pathways and Molecular Events 1. Bioluminescence resonance energy transfer (BRET) Imaging in Plant Seedlings and Mammalian Cells Mohammed Soutto, Qiguang Xie, Xiaodong Xu, Yunfei Zhang, and Carl Hirschie Johnson 2. Luciferase Protein Complementation Assays for Bioluminescence Imaging of Cells and Mice Gary Luker and Kathryn Luker 3. Hybrid Raman-Fluorescence Microscopy on Single Cells using Quantum Dots Henk-Jan van Manen and Cees Otto 4. Labeling of Mesenchymal Stem Cells with Bioconjugated Quatum Dots Bhranti S. Shah and Jeremy J. Mao 5. Quantification of miRNA abundance in single cells using locked nucleic acid-FISH and enzyme labeled fluorescence Jing Lu and Andrew Tsourkas Imaging in Pre-clinical settings 6. Imaging Fate of Stem Cells at a Cellular Resolution in the Brains of Mice Khalid Shah 7. Magnetic Resonance Imaging of Brain Inflamation using Microparticles of Iron Oxide Martina A. McAteer, Constantin von Zur Muhlen, Daniel C. Anthony, Nicola R. Sibson, Robin P. Choudhury 8. Optical Characterization of Arterial Apoptosis Maarten F. Corsten and Abdelkader Bennaghmouch 9. Intravital Near-Infrared Fluorescence Molecular Imaging of Atherosclerosis Farouc Jaffer 10. MR Imaging of Transplanted Stem Cells in Myocardial Infarction Dara L. Kraitchman, Dorota A.. Kedziorek and Jeff W.M. Bulte 11. In Vivo Imaging of the Dynamics of Different Variants of EGFR in Glioblastomas Khalid Shah 12. Fluorescence lifetime-based optical molecular imaging Anand T.N. Kumar Imaging in Clinical Settings 13. PET maging of avb3 Integrins in Cancer Patients Ambros J. Beer and Markus Schwaiger 14. Quantitative Approaches to Amyloid Imaging Victor L. Villemagne, Graeme O’Keefe, Rachel S. Mulligan and Christopher C. Rowe 15. Molecular imaging of myocardial remodeling after infarction Johan W. H. Verjans, Susanne W. M. van de Borne, Leo Hofstra and Jagat Narula 16. Dual-radionuclide Brain SPECT for the Differential Diagnosis of Parkinsonism Georges El Fakhri and Jinsong Ouyang
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