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The study of mental health disorders and the genetics behind these disorders can be greatly enhanced by the use of induced pluripotent stem cells (iPSC). Since many mental health disorders develop after puberty, the only way in which to study the genetic mechanism of these diseases previously was through cellular surrogates, such as blood or cultured fibroblasts. Having the ability to reprogram adult cells to the pluripotent stage provides the capacity to study the onset of these disorders during a culture model of neural development and to include the impact of genetic risk factors and potential environmental triggers. Working with the National Institute of Mental Health (NIMH), the Rutgers Cell and DNA Repository (RUCDR) has begun banking iPSC source cells and converting those source cells into iPSC for distribution to the scientific community. Although initial protocols were developed to reprogram fibroblasts, the ability to reprogram blood cells has several advantages including less invasive collection, less post collection manipulation, and the large number of samples in existing collections. Here, we provide detailed protocols for reprogramming either fibroblasts with retroviral vectors or cryopreserved lymphocytes with Sendai viral vectors. Our goal is to support the discovery of effective treatments for mental health disorders.
Table of Contents: Acknowledgments / Introduction / Appendix / General Protocols / References / Author Biographies
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Biobanking in the Era of the Stem Cell: A Technical and Operational Guide (Colloquium Series on Stem Cell Biology)
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Morgan & Claypool Life Sciences, 2012
ISBN 10: 1615044728
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Biobanking in the Stem Cell Era
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Biobanking in the Era of the Stem Cell
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Condizione: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Working with the National Institute of Mental Health (NIMH), the Rutgers Cell and DNA Repository (RUCDR) has begun banking iPSC source cells and converting those source cells into iPSC for distribution to the scientific community. This book provides detaile. Codice articolo 448166681
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Biobanking in the Era of the Stem Cell: A Technical and Operational Guide (Colloquium Series on Stem Cell Biology)
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Morgan & Claypool Life Sciences, 2012
ISBN 10: 1615044728
ISBN 13: 9781615044726
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Biobanking in the Stem Cell Era: A Technical and Operational Guide
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ISBN 13: 9781615044726
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Biobanking in the Era of the Stem Cell: A Technical and Operational Guide (Colloquium Series on Stem Cell Biology)
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Morgan & Claypool Life Sciences, 2012
ISBN 10: 1615044728
ISBN 13: 9781615044726
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Biobanking in the Era of the Stem Cell: A Technical and Operational Guide (Colloquium Series on Stem Cell Biology)
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ISBN 13: 9781615044726
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Biobanking in the Era of the Stem Cell : A Technical and Operational Guide
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Taschenbuch. Condizione: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - The study of mental health disorders and the genetics behind these disorders can be greatly enhanced by the use of induced pluripotent stem cells (iPSC). Since many mental health disorders develop after puberty, the only way in which to study the genetic mechanism of these diseases previously was through cellular surrogates, such as blood or cultured fibroblasts. Having the ability to reprogram adult cells to the pluripotent stage provides the capacity to study the onset of these disorders during a culture model of neural development and to include the impact of genetic risk factors and potential environmental triggers. Working with the National Institute of Mental Health (NIMH), the Rutgers Cell and DNA Repository (RUCDR) has begun banking iPSC source cells and converting those source cells into iPSC for distribution to the scientific community. Although initial protocols were developed to reprogram fibroblasts, the ability to reprogram blood cells has several advantages including less invasive collection, less post collection manipulation, and the large number of samples in existing collections. Here, we provide detailed protocols for reprogramming either fibroblasts with retroviral vectors or cryopreserved lymphocytes with Sendai viral vectors. Our goal is to support the discovery of effective treatments for mental health disorders. Table of Contents: Acknowledgments / Introduction / Appendix / General Protocols / References / Author Biographies. Codice articolo 9781615044726
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Biobanking in the Era of the Stem Cell: A Technical and Operational Guide (Colloquium Series on Stem Cell Biology)
Editore:
Morgan & Claypool Life Sciences, 2012
ISBN 10: 1615044728
ISBN 13: 9781615044726
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Condizione: New. Codice articolo ABLING22Oct2018170201424
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