Biological membranes play a central role in cell structure, shape and functions. However, investigating the membrane bilayer has proved to be difficult due to its highly dynamic and anisotropic structure, which generates steep gradients at the nanometer scale. Due to the decisive impact of recently developed fluorescence-based techniques, tremendous advances have been made in the last few years in our understanding of membrane characteristics and functions. In this context, the present book illustrates some of these major advances by collecting review articles written by highly respected experts. The book is organized in three parts, the first of which deals with membrane probes and model membranes. The second part describes the use of advanced quantitative and high-resolution techniques to explore the properties of biological membranes, illustrating the key progress made regarding membrane organization, dynamics and interactions. The third part is focused on the investigation of membrane proteins using the same techniques, and notably on the membrane receptors that play a central role in signaling pathways and therapeutic strategies. All chapters provide comprehensive information on membranes and their exploration for beginners in the field and advanced researchers alike.
Le informazioni nella sezione "Riassunto" possono far riferimento a edizioni diverse di questo titolo.
From the reviews:
“The book covers all major applications of fluorescence to membrane studies. ... The book is addressed to scientists and PhD students using fluorescence methods to study biological membranes and membrane proteins. This is a highly interdisciplinary field, and the book will therefore be of interest to biophysicists, physical chemists, and cellular biologists ... . broad overview of different techniques provided by the book is also a good starting point for newcomers to the field. ... a valuable resource for everyone studying membranes and membrane proteins.” (Lorenzo Stella, Analytical and Bioanalytical Chemistry, May, 2013)Part I Fluorescence Probes and Model Membranes
1 Laurdan Fluorescence Properties in Membranes : A Journey from the Fluorimeter to the Microscope
L.A. BAGATOLLI
2 Application of NBD-labeled Lipids in Membrane and Cell Biology
S. HALDAR and A. CHATTOPADHYAY
3 3-Hydroxychromone Probes Precisely Located and Oriented in Lipid Bilayers: a Toolkit for Biomembrane Research
A.S. KLYMCHENKO, G. DUPORTAIL and Y. MÉLY
4 Lateral Membrane Heterogeneity Probed by FRET Spectroscopy and Microscopy
L.M.S. LOURA and M. PRIETO
5 FRET Analysis of Protein-Lipid Interactions
G. GORBENKO and P.K.J. KINNUNEN
6 Hydration and Mobility in Lipid Bilayers Probed by Time-Dependent Fluorescence Shifts
Š. POKORNA, A. OLŻYŃSKA, P. JURKIEWICZ* and M. HOF
Md. JAFURULLA and A. CHATTOPADHYAY
Part II Exploring Membrane Organization, Dynamics and Interactions by Advanced Fluorescence-Based Imaging Techniques
7 Visual Discrimination of Membrane Domains in Live Cells by Wide-Field Microscopy
C.E. BUTLER, G. WHEELER, J. GRAHAM and K.M. TYLER
8 Quantitative Fluorescence Studies of Intracellular Sterol Transport and Distribution
D. WÜSTNER, F.W. LUND and L.M. SOLANKO
9 Studying Membrane Properties Using Fluorescence Lifetime Imaging Microscopy (FLIM)
M.T. STÖCKL, R. BIZZARRI and V. SUBRAMANIAM
10 Fluorescence Correlation Spectroscopy to Study Membrane Organization and Interactions
M. ZELMAN-FEMIAK, Y. SUBBURAJ and A.J. GARCIA-SÁEZ
11 Deciphering Cell Membrane Organization Based on Lateral Diffusion Measurements by Fluorescence Correlation Spectroscopy at Different Length Scales
V. ROUGER, C. BILLAUDEAU, T. TROMBIK, S. MAILFERT, Y. HAMON, H.-T. HE and D. MARGUET
12 STED-FCS Nanoscopy of Membrane Dynamics
C. EGGELING
13 Imaging Molecular Order in Cell Membranes by Polarization Resolved Fluorescence Microscopy
S. BRASSELET, P. FERRAND, A. KRESS, X. WANG, H. RANCHON and A. GASECKA
14 Near-Field Optical Nanoscopy of Biological Membranes
T.S. van ZANTEN, C. MANZO and M.F. GARCIA-PARAJO
Part III Characterization of Membrane Proteins and Receptors by Advanced Fluorescence-based Imaging Techniques
15 Unveiling Biophysical and Biological Properties of a Hypothetical Membrane receptor by Exploiting recent Imaging Advances
P. GONNORD and R. VARMA
16 New Fluorescent Strategies Shine Light on the Evolving Concept of GCPR Oligomerization
M. COTTET, O. FAKLARIS, E. TRINQUET, J.-P. PIN and T. DURROUX
17 Application of Quantitative Fluorescence Microscopic Approaches to Monitor Organization and Dymamics of the Serotonin1A Receptor
Md. JAFURULLA and A. CHATTOPADHYAY
18 TNF Receptor Membrane Dynamics Studied with Fluorescence Microscopy and Spectroscopy
F. NEUGART, D. WIDERA, B. KALTSCHMIDT, C. KALTSCHMIDT and M. HEILEMANN
19 HIV-1 Gag Directed Assembly of Retroviral Particles Investigated by Quantitative Fluorescence Imaging
H. de ROCQUIGNY, H. GACEM, P. DIDIER, J. L. DARLIX and Y. MÉLY
Le informazioni nella sezione "Su questo libro" possono far riferimento a edizioni diverse di questo titolo.
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Descrizione libro Soft Cover. Condizione: new. Codice articolo 9783642441073
Descrizione libro Condizione: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Written by experts in the fieldRichly illustrated An invaluable source of information for beginners in the field and advanced researchers Biological membranes play a central role in cell structure, shape and functions. . Codice articolo 5061107
Descrizione libro Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Biological membranes play a central role in cell structure, shape and functions. However, investigating the membrane bilayer has proved to be difficult due to its highly dynamic and anisotropic structure, which generates steep gradients at the nanometer scale. Due to the decisive impact of recently developed fluorescence-based techniques, tremendous advances have been made in the last few years in our understanding of membrane characteristics and functions. In this context, the present book illustrates some of these major advances by collecting review articles written by highly respected experts. The book is organized in three parts, the first of which deals with membrane probes and model membranes. The second part describes the use of advanced quantitative and high-resolution techniques to explore the properties of biological membranes, illustrating the key progress made regarding membrane organization, dynamics and interactions. The third part is focused on the investigation of membrane proteins using the same techniques, and notably on the membrane receptors that play a central role in signaling pathways and therapeutic strategies. All chapters provide comprehensive information on membranes and their exploration for beginners in the field and advanced researchers alike. 500 pp. Englisch. Codice articolo 9783642441073
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Descrizione libro Taschenbuch. Condizione: Neu. Druck auf Anfrage Neuware - Printed after ordering - Biological membranes play a central role in cell structure, shape and functions. However, investigating the membrane bilayer has proved to be difficult due to its highly dynamic and anisotropic structure, which generates steep gradients at the nanometer scale. Due to the decisive impact of recently developed fluorescence-based techniques, tremendous advances have been made in the last few years in our understanding of membrane characteristics and functions. In this context, the present book illustrates some of these major advances by collecting review articles written by highly respected experts. The book is organized in three parts, the first of which deals with membrane probes and model membranes. The second part describes the use of advanced quantitative and high-resolution techniques to explore the properties of biological membranes, illustrating the key progress made regarding membrane organization, dynamics and interactions. The third part is focused on the investigation of membrane proteins using the same techniques, and notably on the membrane receptors that play a central role in signaling pathways and therapeutic strategies. All chapters provide comprehensive information on membranes and their exploration for beginners in the field and advanced researchers alike. Codice articolo 9783642441073
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