Cytoskeletal and Extracellular Proteins: Structure, Interactions and Assembly the 2nd International Ebsa Symposium: 3 - Brossura

Brossura

ISBN 10: 364273927X ISBN 13: 9783642739279

Casa editrice: Springer Nature, 2011

Riassunto

In this volume the contributions of the 2nd International EBSA (European Biophysical Societies Association) Symposium devoted to the biophysical and biochemical aspects of the structure and interaction of cytoskeletal and extracellular proteins are presented. Topics such as supramolecular structure and organization, thermodynamics and kinetics of assembly, as well as the basic mechanisms of protein-protein interactions are discussed, and special emphasis is given to applied biophysical techniques.

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Contenuti:

SECTION I: Cytoskeletal Proteins and Their Supramolecular Assemblies.- Functional Properties of ?-Tubulin In Vivo.- Paracrystals of Tau Protein ― A Molecular Accordion.- Structure and Assembly of Intermediate Filaments: Multi-Faceted, Myosin-Like (But Non-Motile) Cytoskeletal Polymers.- Structure and Assembly of Calf Hoof Keratin Filaments.- Structure and Assembly of Neurofilaments (NF): Analysis of Native NF and of Specific NF Subunit Combinations.- Mitochondrial Creatine Kinase (Mi-CK) Forms Octameric Molecules: Structure-Function Relationship and Implications for the CP-Shuttle.- Current State of the Structural Analysis of the Actin: DNase I Complex.- The Structure of F-Actin Calculated from X-Ray Fibre Diagrams and the 0.6 nm Crystal Structure.- The “Lateral Slipping” Model of F-Actin Filaments.- A New Model of Actin ― A Rigid Helical Backbone with Flexible Outer Domains.- Biochemical and Structural Analysis of the Interaction of a-Actinin with Actin Filaments and Lipids.- Functional Analysis of Actin Binding Proteins In Vitro and In Vivo.- Calcium- and Actin-Binding Sites in F-Actin Crosslinking Molecules from Dictyostelium Discoideum.- Comparison of Various Gelsolin-Like Proteins and Their Interaction with Actin Filaments.- SECTION II: Components and Supramolecular Assemblies of the Extracellular Matrix.- Basement Membrane (Type IV) Collagen ― Its Molecular and Macromolecular Structure.- Effects of Mutations that Change Primary Structure of Collagen on the Self-Assembly of the Protein into Fibrils.- Molecular Cloning of Chicken Type VI Collagen.- Structure and Biology of the Laminin-Nidogen Complex.- Structure of the Basement Membrane Protein Laminin: Variations on a Theme.- Triple Coiled-Coil Regions of Laminin: Specificity and Chain Stability.- A Fragment of Laminin Comprising the Entire Long Arm.- 5?-Nucleotidase as Receptor for the Extracellular Matrix Proteins Laminin and Fibronectin.- Monoclonal Antibody 127 Recognizes a Subpopulation of Chicken Hexabrachion/ Tenascin.- Functional Domains of Tenascin.- Complex Formation of Endothelial Cell Thrombospondin with Heparin and Fibronectin.- Functional Aspects of Proteoglycan Domain Structure.- SECTION III: Structural Motifs of Multi-Domain Proteins.- Multi-Domain Proteins: Towards Complete Structures.- Structure and Spatial Organisation of Intermediate Filament and Nuclear Lamin Molecules.- The Role of Repeating Sequence Motifs in Interactions Between a-Helical Coiled-Coils such as Myosin, Tropomyosin and Intermediate-Filament Proteins.- The Self-Assembly of Nonsarcomeric Myosins.- Shape Changes in Myosin Induced by the Regulatory Light Chain Subunits.- Plectin/Vimentin Interaction: Molecular Binding Domains and Regulation by Phosphorylation.- Interaction of Phosphoproteins with Calcium Phosphate.- Thermal Stability and Folding of the Triple Helices of Interstitial and Basement Membrane Collagens.- Refolding of Collagen IV and Other Collagens as Monitored by Electron Microscopy.- SECTION IV: Biophysical Methods for Elucidation of the Structure and Assembly of Cytoskeletal and Extracellular Constituents.- Scanned and Fixed Beam Microscopy of Cytoskeletal Components.- Diffraction Studies of Glycated Collagen.- Oscillations in Microtubule Assembly Studied by Time-Resolved X-Ray Scattering.- Study of the Secondary Structure of Tubulin in Solution and in Microtubules by Raman Spectroscopy.- Light Scattering Techniques.- Chain Dynamics, Transport and Structural Properties of Actin Networks: A Quasielastic Light Scattering and Microfluorescence Study.- Polymerization of Actin on Supported Lipid Bilayers.- Macrophage Intracellular Motility and Its Correlation with Cell-Substrate Adhesion.- Adhesion of Pulmonary Macrophages to Langmuir-Blodgett Films, Investigated by Interference Reflection Microscopy.- SECTION V: Thermodynamics and Kinetics of Assembly.- Microtubule Dynamics: Experimental Evidence and Numerical Modelling.- The Effects of Magnesium on the Dynamic Instability of Individual Microtubules.- About the Recognition of Colchicine by Tubulin.- Phosphate Release Following Nucleotide Hydrolysis Regulates the Dynamics of Actin Filaments and Microtubules.- Self-Assembly of Supramolecular Structures: The Role of Fluctuations.- Structure-Function Studies of the Actin Filament System of Acanthamoeba.- Equilibrium and Kinetics of the Assembly of Capping Proteins with the Ends of Actin Filaments.- Lithium, Thiocyanate and Actin Assembly.- Procollagen Processing Control of Type I Collagen Fibril Assembly.- Supramolecular Organization of Extracellular Matrices: Analogy with Liquid Crystals.- SECTION VI: Complex Supramolecular Structures.- A Modular Model of the Nuclear Pore Complex.- Maps in all Varieties ― The Cytoskeleton of Trypanosoma Brucei.- Isolation and Partial Characterization of Ciliary Rootlets from Paramecium Tetraurelia.- Strategies in Regulation of Protein Associations of the Spectrin-Based Membrane Skeleton.- A cAMP-Dependent Phosphorylation Alters Spectrin Binding Properties.- Inhibition of Tropomyosin Binding to F-Actin by Tropomodulin, a New Tropomyosin-Binding Protein from the Human Erythrocyte Membrane.- A Model for Protein-Protein Interactions Involved in the Linkage of the Actin Cytoskeleton to Transmembrane Receptors for Extracellular Matrix Proteins.- The Anchorage of the Cytoskeleton to the Plasma Membrane: Interaction of Vinculin with Membranes In Vitro and In Situ.- Connectors of Supramolecular Assemblies.- Basement Membrane Synthesis in Drosophila: Indications for more than One Assembly Mechanism for Basement Membranes.- Laminin Polymerization and Binding to Glycosaminoglycans: A Hypothesis for Modulation of Basement Membrane Structure.

Su questa edizione

• Editore: Springer Nature
• Data di pubblicazione: 2011
• ISBN 10: 364273927X
• ISBN 13: 9783642739279
• Rilegatura: Copertina flessibile
• Numero di pagine: 380
• Redattore: Aebi Ueli, Engel Jrgen