Monoclonal antibodies anti-beta2-glycoprotein I: Production, characterization, analytical applications - Brossura

Stella, Carolina Nigro; Ferreira Gomes, Lígia

Brossura

ISBN 10: 3659393851 ISBN 13: 9783659393853

Casa editrice: LAP LAMBERT Academic Publishing, 2013

Riassunto

Beta2-glycoprotein I (β2GPI) detection is important for the accurate diagnosis of autoimmune and chronic inflammatory disease. This book describes the main steps towards a successful obtaining, initial characterization, analytical applications and lab-scale production of a set of anti-β2GPI monoclonal antibodies. The β2GPI antigen purification was comprehensively explored and six different hybridomas were obtained after cell fusion of immunized mouse lymphocytes with SP2-O murine myeloma cells. The antibodies were purified after hybridomas expansion from ascites (in vivo) and conventional cell culture (in vitro) and their reactivity was evaluated towards the β2GPI antigen either adsorbed to solid phase support (Dot-Blot, immunoblotting, direct ELISA), or in solution (indirect ELISA). β2GPI recognition was achieved with one of the clones in paraffin embedded tissues (immunohistochemistry), and in blood and lymph (ELISA). The application of β2GPI recognition to a sepsis and bacterial translocation animal model granted an unexpected and exciting sequence of achievements concerning the pathophysiology of autoimmunity control by this protein.

Su questo libro

L'autore:

MSc in Pharmaceutical Sciences (Clinical Chemistry) from University of São Paulo (USP), cytologyst and faculty member at Faculdades Oswaldo Cruz, Centro Universitário São Camilo and Universidade Paulista. Lígia Ferreira Gomes is PhD in Biochemistry and tutored this work at Faculty of Pharmaceutical Sciences - USP, Brasil

Su questa edizione

• Editore: LAP LAMBERT Academic Publishing
• Data di pubblicazione: 2013
• ISBN 10: 3659393851
• ISBN 13: 9783659393853
• Rilegatura: Copertina flessibile
• Numero di pagine: 92