Miniaturization and Mass Spectrometry

Severine le Gac

ISBN 10: 085404129X / ISBN 13: 9780854041299

Editore: Royal Society of Chemistry, 2008

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Riassunto: The recent explosion in the use of analytical chemistry, particularly in the biological sciences, has led to a need for fast, reliable and highly sensitive tools able to handle small sample sizes. This book illustrates how microfluidics and lab-on-a-chip devices can satisfy the growing need for miniaturized and enhanced analysis. They lend themselves well to mass spectrometric detection as they use samples in the low microlitre range and are handled on a chip. Miniaturization and Mass Spectrometry focuses on one particular technique, mass spectrometry, whose popularity has increased dramatically in the last two decades with the increase in use of biological analysis and the development of two "soft" ionization techniques, ESI and MALDI. These enable the analysis of large but fragile biological molecules such as DNA, proteins and oligosaccharides. The book starts with an introduction to the coupling of microfluidics to mass spectrometry techniques. It then goes on demonstrate the advantages of such a coupling: the MS analysis benefits from improved sample preparation when performed on a chip while MS yields more information on the sample handled on the chip compared to conventional optical detection. A history on the developments in this field, starting from the off-chip coupling to the on-chip ionization, is also provided. Daniel Figeys, a pioneer in the development of microfluidic systems for MS analysis, describes the early beginnings of this hyphenated analysis technique. Solutions to couple microfluidic systems to the two most popular ionization methods, ESI and MALDI, are presented throughout the chapters. Various examples are given of the application of this microfluidics-MS hyphenated analysis technique to proteomics, metabolomics, organic chemistry and forensics. Coverage is not limited to academic research. The development of commercialized systems and their current use for routine biological analysis are also presented. Lastly, a future vision of the integration of the mass spectrometer on the chip is raised, as a last step to yield fully portable systems for on-site analysis.

Book Description: The explosion in the late 20th century of the field of analytical chemistry and particularly for biological purposes- has led to a need for fast, integrated, portable, more reliable and more sensitive tools that could handle reduced amount of samples. Microfluidics and lab-on-a-chip devices provide a good answer to this quest to miniaturized and enhanced analysis and they lend themselves well to mass spectrometric detection. This book illustrates this trend and discusses the analysis of large but frail molecules, e.g DNA, proteins and oligosaccharides. Essential reading for anyone with an interest in this field.

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Titolo: Miniaturization and Mass Spectrometry
Casa editrice: Royal Society of Chemistry
Data di pubblicazione: 2008
Legatura: Hardcover
Condizione libro: New