humana press mrz 2012 (30 risultati)

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Microelectronic engineering has revolutionized electronics, providing new, faster and cheaper ways of doing things - and now the same technology is being applied to biotechnology and molecular biology. As sample volume is reduced, reaction speed and detector sensitivity are increased whilst sample and reagent requirements and device cost are reduced. Microelectronic engineering provides the potential for bench-top versions of large and expensive equipment such as flow cytometry, or novel ones that exploit physical phenomena on the micron scale, such as dielectrophoresis for cell analysis.In Microengineering in Biotechnology, experts in the field contribute chapters aimed at instilling in the reader a working understanding of the methods underlying microengineering and the means by which such methods can be used for a range of analytical techniques. It describes the methods by which microengineered devices can be built to perform a number of applications and considers how the field may progress by examining some more complex lab on a chip devices which have great potential in the advancement of the way in which molecular biology is performed. As a volume in the highly successful Methods in Molecular Biology(TM) series, this work provides the kind of detailed description and implementation advice that is crucial for getting optimal results.Cutting-edge yet easy-to-use, Microengineering in Biotechnology serves as a reference guide for practical microengineering techniques and as a route into the development of new devices for biological applications in order to strengthen the promising union of molecular and cellular biology with microelectronics. 264 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -As two of the leading causes of death worldwide, heart disease and stroke represent a clear target for genomic research aimed at deciphering the genes and cellular pathways that underlie cardiovascular disease and creating improved therapies. In Cardiovascular Genomics: Methods and Protocols, experts in the field provide methods for cardiovascular phenotyping of rodent models in the first section of the volume and for statistical and bioinformatic integration of phenotype data with genome-wide genotype and expression data in the second section. Understanding these diverse methods will allow an individual laboratory to utilize these genomic methods independently or to better prepare for collaboration with scientists having expertise in other disciplines in order to uncover genes affecting cardiovascular disease. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary equipment, materials, and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Cutting-edge and easy-to-use, Cardiovascular Genomics: Methods and Protocols will enable researchers to identify causal genes and novel molecular targets that can lead to vital new treatments for cardiovascular disease. 360 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Due to their versatility, along with the diminishing costs of library synthesis and the growth of commercial support, peptide microarrays will likely expand beyond being just a research tool into an adaptable and powerful platform to be harnessed for wider drug discovery and point-of-care applications. In Peptide Microarrays: Methods and Protocols, experts in the field provide a cutting-edge view of peptide array technology, its applications, and technical issues. After examining how peptides can characterize proteins and clarify, at the amino acid level, the molecular recognition events in which they are involved, the volume goes on to cover topics such as the production and use of peptide arrays for enzyme and binding motifs characterization, epitope mapping, and diagnostics, the newest technological advancements, as well as software and web tools for the design of peptide arrays and for the analysis of output data. As a volume in the highly successful Methods in Molecular Biology(TM) series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and easy-to-use, Peptide Microarrays: Methods and Protocols promises to serve scientists with its unique insights and novel solutions in peptide array technology in order to advance this vital field. 448 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -In Bladder Cancer: Moving the Field Forward, Cheryl Lee, David Wood, and a panel of leading authorities and researchers provide a comprehensive review of the related literature, while providing insights into the obstacles of improved survival and discuss methods to advance the field of Bladder Cancer care. Their work aims to spur innovative thoughts and approaches to common problems in the management of early stage and advanced disease. The book discusses at length the state of bladder cancer staging and the optimal treatment for localized disease. Additional sections review the applications of existing systemic therapies and look at the infrastructure needed to support translational research efforts. Groundbreaking and authoritative, Bladder Cancer: Moving the Field Forward, is the definitive text on the current state of bladder cancer care and a must have for all urologists and medical oncologists who wish to provide their patients with the most comprehensive care. 348 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Stem Cell Therapy for Diabetes, one of the latest installments of the Stem Cell Biology and Regenerative Medicine series, reviews the three main approaches for generation of sufficient numbers of insulin-producing cells for restoration of an adequate beta-cell mass: beta-cell expansion, stem-cell differentiation, and nuclear reprogramming. Adeptly collecting the research of the leading scientists in the field, Stem Cell Therapy for Diabetes compares the merits of employing autologous versus banked allogeneic cell sources for generation of surrogate beta cells, and addresses tissue engineering and ways for cell protection from recurring autoimmunity and graft rejection. Stem Cell Therapy for Diabetes provides essential reading for those especially interested in tracking the progress in applying of one of the most exciting new developments in bio-medicine towards a cure for diabetes. 304 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Due to their importance to cell biology, DNA topology-altering enzymes, collectively DNA topoisomerases and gyrases, have been studied thoroughly from a biochemical perspective, yet the expansion of research at the molecular and cellular levels continues to show a great deal of promise. In DNA Topoisomerases: Methods and Protocols, experts in the field present a compendium of protocols that extends into the new areas of research that have been recently uncovered in order to elucidate the methodology needed to study topoisomerases in the molecular and cellular context. The volume examines subjects such as the analysis of DNA topologies and the interaction of topoisomerases with DNA as tools to measure topoisomerase functions, the binding of topoisomerases to specific sites in the genomes of eukaryotes, including origins of DNA replication, as well as exploration of perturbed topoisomerase function. As a volume in the highly successful Methods in Molecular Biology(TM) series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and notes on trouble-shooting and avoiding known pitfalls.Cutting-edge and easy-to-use, DNA Topoisomerases: Methods and Protocols provides key content which reflects an expanded understanding of the functions of these essential enzymes in the hope of inspiring researchers to further important discoveries in this dynamic field. 280 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -The renin angiotensin system plays an essential role on cardiac and vascular functions and their connection in the generation of cardiovascular diseases. Renin Angiotensin System and Cardiovascular Disease covers indispensable aspects such as intracellular signaling, regulation of cell volume in the failing heart, the presence of an intracrine renin angiotensin system, and the influence of prorenin/renin, angiotensin II, angiotensin (1-7) and aldosterone. Furthermore, the role of the mineralocorticoid receptor as an imperative component of the intracrine renin angiotensin system and as a regulator of extracellular action of angiotensin II is described, reinforcing the view that aldosterone inhibitors are helpful in the treatment of heart failure and hypertension. Renin Angiotensin System and Cardiovascular Disease provides readers with an outstanding review of the major topics involving the renin angiotensin system and cardiovascular disease. With contributions by an international panel of experts, Renin Angiotensin System and Cardiovascular Disease presents the most relevant aspects of the role of the renin angiotensin aldosterone system on cardiovascular diseases including hypertension, congestive heart failure, atherosclerosis and diabetes. The volume Editors, Dr. Walmor C. DeMello and Dr. Edward Frohlich, both established specialists in their respective fields, team up to discuss a range of topics from Renin Angiotensin Aldosterone System and Pathobiology of Hypertension to Inhibiting the Renin Angiotensin Aldosterone System in Patients with Heart Failure and Myocardial Infarction. 260 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Part I I wasraised in a redbrick Baltimore row housewhere summer was marked by the ti- honored ritual of firefly-chasing - a backyard tradition that has endured the gene- tions.Amidtheexcitement,myfatheroftentoldthestoryofhow,whenh ewasachild, researchers at the Johns Hopkins University had appealed for the systematic capture of live fireflies en masse. Science had engaged the Baltimore youth in an entrepreneurial quest to jar as many lightning bugs as the dwindling light of dusk would permit. The very next morning, each 100-count glass jar of glowing crawling insects could be exchanged at the University for exactly one crisp dollar bill. Unrecognized at the time by my father, his joyous endeavors had contributed in a profoundwaytotheadvancedmolecularbiologicaltechniquesthatserveasthebasisfor thistextbook.In1947,WilliamMcElroyusedextractsfromthoseveryfirefliestodefine the fundamental reaction underlying the mystical phenomenon of luminescence, and published ''The energy source for bioluminescence in an isolated system'' in the Proceedings of the National Academy of Sciences. Inthedecadessincethatsummer,thestudyandapplicationofbioluminescencehave allowed us to leverage the enduring power of nature's elegance. We have painstakingly harnessedapowerfultoolthatenablesustoseekadeeperunderstandingo fthecomplex mechanisms underpinning so many vital biologic systems. This second edition of Methods in Molecular Biology's Bioluminescence: Methods and Protocols serves as a re- ableandutilitariancompilationofthenewestandmostinnovativetechni questhathave emerged in this rapidly expanding and progressively diverse field. We are indebted to the authors for their thoughtful contributions, inspired by their rigorous dedication to the science of bioluminescence, humbled by the unyielding supportofourcolleagues,andgratefulfortheopportunityprovidedusbyJohnandJan Walker. 280 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Fundamental to the development and vital functions of organisms, the migration of motile cells due to the detection of shallow gradients of specific chemical signals in their environments, or chemotaxis, can be clearly seen as a major force in cell biology. In Chemotaxis: Methods and Protocols, expert researchers in the field provide state-of-the-art methods for investigating cell migration behaviors, studying molecular components involved in detecting extracellular signals and directing cell movement, visualizing spatiotemporal dynamics of the components in signaling networks of chemotaxis in real time, and constructing quantitative models that simulate chemoattractant-induced cell responses. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, Chemotaxis: Methods and Protocols serves scientists with practical guidance on the diverse methodologies that are currently propelling chemotaxis research forward in order to further our understanding of this vital biological system. 556 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -While ornamental plants are produced mainly for their aesthetic value, the propagation and improvement of quality attributes such as leaf types, flower colour and fragrance, longevity and form, plant shape and architecture, and the creation of novel variation are important economic goals for the expanding ornamental industry. In Protocols for In Vitro Propagation of Ornamental Plants, leading researchers in the field compile step-wise protocols for rapid plant multiplication and in vitro storage of major commercially viable ornamental plants. Divided into two sections, Section A contains chapters mainly on micropropagation of cut and pot flowers with detailed protocols involving in vitro culture-explants, medium preparation, detailed medium table, shoot initiation and proliferation, root induction, in vitro plant hardening, and field transfer, and Section B delves into reviews on topics such as in vitro production of sweet peas, the status of transgenics in ornamental plants, in vitro conservation, the status of floriculture in Europe, azalea phylogeny, and thin cell layers. As part of the highly successful Methods in Molecular Biology(TM) series, chapters include introductions to their respective topics, lists of the necessary materials, step-by-step laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Comprehensive and clear, Protocols for In Vitro Propagation of Ornamental Plants presents key techniques that will be of great use to floriculturists, researchers, commercial companies, biotechnologists, and students dealing with ornamental plants. 416 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -In recent years, much information has been revealed concerning the essential role of helicases, the enzymes that utilize the energy derived from nucleoside triphosphate hydrolysis to unwind the double stranded helical structure of nucleic acids. In Helicases: Methods and Protocols, this topic is explored by leading international scientists who have contributed significantly to our current understanding of helicases and the ways in which they function. Chapters include methods for the production and purification of helicases from different species, as well as detailed studies of helicase activities, including NTP binding and hydrolysis, nucleic acid binding and unwinding, and translocation along nucleic acid substrates. Composed in the highly successful Methods in Molecular Biology(TM) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.Wide-ranging and innovative, Helicases: Methods and Protocols is the ultimate guide for researchers, providing the scientific community with comprehensive accounts of the most advanced methods used to study helicases today. 416 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Chemogenomics aims toward the systematic identification of small molecules that interact with the products of the genome and modulate their biological function. The establishment, analysis, prediction, and expansion of a comprehensive ligand-target SAR (structure-activity relationship) matrix has followed the elucidation of the human genome and presents a key scientific challenge for the twenty-first century. The anno- tion and knowledge-based exploration of the ligand-target SAR matrix is then expected to impact science greatly. Progress alongside this challenge without a doubt will c- tribute to further the fundamental understanding of the biological function of the individual proteins and ultimately provide a basis for the discovery of new and better therapies for diseases. While historically the chemogenomics approach is based on efforts that systematically explore target gene families, today broader in vitro and in silico approaches are available to encompass wider genomes. In this book, experts from academia and industry outline relevant aspects of ch- istry, biology, and molecular informatics which are the cornerstones of chemogenomics. General introductory chapters are combined with chapters describing methods and pro- cols, which are the gold standard of the Methods in Molecular Biology book series. 332 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Over the past twenty years, the development of chromatin immunoprecipitation, or ChIP, assays has immensely enhanced the biological significance of the multifaceted DNA-binding proteins. In Chromatin Immunoprecipitation Assays: Methods and Protocols, researchers deeply involved in the development and improvement of the field provide cutting-edge protocols devoted to the most recent progress in ChIP and related subjects. The thorough chapters involve topics such as the characterization of ChIP antibodies, ChIP methods for small cell numbers, fast ChIP protocols, assays adapted to various species and cell types, as well as several strategies for the analysis of genome-wide datasets, while also extending a bit beyond ChIP assays to cover immunoprecipitation-based DNA methylation analyses, determination of spatial chromatin organization of large genomic regions, and RNA immunoprecipitation. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters contain brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and easy-to-use, Chromatin Immunoprecipitation Assays: Methods and Protocols is a timely guide for all those who wish to further the development of this powerful tool and continue delving into the incredible complexity of the cell's biology. 280 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This editor believes the public finally understands that bioenergy should be a critical new 39 component of our world's future energy supply. While this new enlightenment might have 40 come from bioenergy's environmentally friendly attributes and its renewable and sustain- 41 able nature, the reality is that the primary driver has been both the high cost of energy, 42 led recently by petroleum products, and the unique climatic changes such as local weather 43 events and reports of melting of ancient glaciers. I believe that much of the public under- 44 stands the primary cause of these changes is the growing demand for fossil fuels for heat- 45 ing and transportation needs driven by both population increases and dramatic economic 46 development around the globe. This increased demand, as well as global politics, has 47 impacted the costs for these energy products. At the same time, increased use of fossil fuels 48 has been liberating unprecedented levels of CO and other greenhouse gasses (GHGs) 49 2 that must be impacting the global climate. As a result, the world is looking for alternative 50 solutions. Renewable energy is one of the leading options as long as it is pursued in a sus- 51 tainable fashion. Of course, renewable energy has numerous sources, including wind, solar, 52 geothermal, ocean currents/tides, and bio-based energy. The latter category of renewable 53 energy is centered primarily on use of green plant matter, i. e. 308 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Affectingapproximatelytwenty percentof the world population, mood and anxiety disorders have been the subject of ever-increasing research. This increased research parallels a remarkable growth in the use of the laboratory mouse as a tool to understand the biological and genetic basis of mood and anxiety disorders as well as to develop improved treatments. In Mood and Anxiety Related Phenotypes in Mice: Characterization Using Behavioral Tests, world-renowned researchers provide an overview of behavioral approaches utilized in the characterization of mood and anxiety related behaviors in mice as well as commonly used behavioral assays to assess the potential antidepressant and anxiolytic efficacy of novel compounds. As a volume in the successful Neuromethods(TM) series, the chapters provideauthoritative reviews including up-to-date detailed protocols of the most commonly used approaches in the field. Mood and Anxiety Related Phenotypes in Mice: Characterization Using Behavioral Tests is an ideal resource for scientists actively pursuing or interested in establishing behavioral protocols in their laboratories, while also serving as a reference for those students, scientists, and practitioners who have an interest in better understanding the preclinical behavioral methods used in mood and anxiety research. 348 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Over the last few years, the methods for analyzing cancer-related genes on a molecular level have changed rapidly. The advent of incredible new technology, such as automated sequencing, has served as a catalyst for many advances in the area of gene profiling, and has enabled the research community to investigate populations of cells in a given tissue. Cancer Gene Profiling: Methods and Protocols examines the world of cancer gene profiling in light of these new advances, providing highly developed microarray techniques alongside modern methods for arraying tissues and proteomics. Chapters bring together the experiences of leading scientists in each method of profiling, providing a clear overview of the ground-breaking techniques used to perform these experiments effectively. Composed in the highly successful Methods in Molecular Biology(TM) series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.Comprehensive and state-of-the-art, Cancer Gene Profiling: Methods and Protocols is a fundamental guidebook for researchers in the field of cancer gene profiling, as well as for those individuals in the area of biomedical/translational research. 464 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -In recent years, our ability to understand and manipulate epidermal cells has increased tremendously, opening significant new possibilities in both basic science research and in regenerative medicine, including would healing and transplantation. Epidermal Cells: Methods and Protocols, Second Edition expands upon the popular first edition by bringing together a panel of experienced basic and clinical researchers to describe in step-by-step detail the powerful methods they have developed and optimized to analyze and manipulate epidermal cell precursors and mature epidermal cells. These protocols cover different methods and models for culturing epidermal cells, for enriching very early epidermal progenitors, as well as for studying epidermal cell commitment and differentiation both in vitro and in vivo. Topics of special interest include the derivation, characterization, and utility of epidermal stem cells, mature epidermal cells and their characterization, and applications in regenerative medicine. The protocols follow the successful Methods in Molecular Biology(TM) series format, providing introductions to their respective topics, lists of the necessary materials and reagents, and notes on troubleshooting and avoiding known pitfalls.State-of-the-art and highly practical, Epidermal Cells: Methods and Protocols, Second Edition offers experienced and novice investigators alike an invaluable collection of readily reproducible techniques designed to broaden not only our understanding of the biology of epidermal cells, but also their utility in normal tissue homeostasis and regenerative medicine applications. 464 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -DNA has been known to be the cellular target for many cytotoxic anticancer agents for several decades. The knowledge of its structure in atomic detail and the ease with which DNA fragments (both synthetic oligonucleotides and natural sequences) can be prepared and manipulated has aided the design of compounds that bind to it with improved sel- tivity. On the basis of this information, new generations of sequence reading compounds (including triplex forming oligonucleotides and minor groove binding ligands) have been prepared, which have the potential for targeting specifc DNA sequences as anti-gene agents. Within the last 10 years, it has also become apparent that the familiar DNA duplex is not the only structure that can be targeted by DNA-binding ligands and there has been increased interest in triplex and quadruplex structures as drug targets, as well as protein- DNA complexes, such as those with nucleosomes or topoisomerases. Each of these advances has required the availability and development of an arsenal of techniques for probing the interactions in both qualitative and quantitative terms. This v- ume of Methods in Molecular Biology brings together several techniques that are currently useful for examining these interactions. Some of these are updates on ones that were included in the earlier volume (Methods in Molecular Biology 90), published 12 years ago, while others are new. 332 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Together with early theoretical work in population genetics, the debate on sources of genetic makeup initiated by proponents of the neutral theory made a solid contribution to the spectacular growth in statistical methodologies for molecular evolution. Evolutionary Genomics: Statistical and Computational Methods is intended to bring together the more recent developments in the statistical methodology and the challenges that followed as a result of rapidly improving sequencing technologies. Presented by top scientists from a variety of disciplines, the collection includes a wide spectrum of articles encompassing theoretical works and hands-on tutorials, as well as many reviews with key biological insight. Volume 1 includes a helpful introductory section of bioinformatician primers followed by detailed chapters detailing genomic data assembly, alignment, and homology inference as well as insights into genome evolution from statistical analyses. Written in the highly successful Methods in Molecular Biology(TM) series format, this work provides the kind of advice on methodology and implementation that is crucial for getting ahead in genomic data analyses. Comprehensive and cutting-edge, Evolutionary Genomics: Statistical and Computational Methods is a treasure chest of state-of the-art methods to study genomic and omics data, certain to inspire both young and experienced readers to join the interdisciplinary field of evolutionary genomics. 484 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -In the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. Recombinant Protein Production in Yeast: Methods and Protocols examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein prior to purification. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant Protein Production in Yeast: Methods and Protocols, seeks to aid scientists in adopting yeast as a protein production host. 260 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Molecular cloning and DNA-based analysis have become part of every molecular life science laboratory. The rapid adoption of DNA-based techniques has been facilitated by the introduction of the polymerase chain reaction (PCR), which has made cloning and characterization of DNA quick and relatively simple. PCR is virtually part of every variation of the plethora of approaches used for DNA fingerprinting today. Plant DNA Fingerprinting: Methods and Protocols aims to bring together the different currently available genome-based techniques into one repository. This volume contains detailed protocols for the preparation of plant genomic DNA, fingerprinting of plants for the detection of intra-species variations, the use of DNA barcoding, as well as methods for the bioinformatic analysis of data. Also included are several discussions on the broader issues of genome-based approaches in order to provide a sound understanding of the principles of these methods. Written in the successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Plant DNA Fingerprinting: Methods and Protocols is tailored principally for those who seek to augment their current methods of plant analysis and quality control using genome-based approaches as well as for scientists and researchers in different plant sciences. 212 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Iron deficiency is ever-present among all populations throughout the world irrespective of race, culture, or ethnic background. Even with the latest advances in medicine, improved nutrition, and the ready availability of cheap oral iron, there is still no satisfactory explanation for the widespread occurrence of iron deficiency or for the absence of an effective treatment. Iron Deficiency and Overload: From Biology to Clinical Medicine is an important new text that provides a timely review of the latest science concerning iron metabolism as well as practical, data-driven options to manage at-risk populations with the best accepted therapeutic nutritional interventions. Chapter topics reflect the excitement in current theoretical development and laboratory activity in this area. The distinguished authors address their presentations to professionals and graduate students who need to be better informed about the concepts, methodologies, and current status of the field. Iron Deficiency and Overload: From Biology to Clinical Medicine is an essential text that presents a sampling of the major issues in iron research, from the most basic research level to human applications. 392 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -'Next generation' sequencing techniques allow for more detailed analysis of exons and introns in multiple genes at the same time. This will reveal many mutations that potentially lead to exon skipping. To functionally test these a lot can be achieved with a limited set of protocols, while for the intentional induction of exon skipping different tools and target genes are involved and the translational path from in vitro splicing to in vivo tests in animal models requiring a more extensive set of protocols. Exon Skipping: Methods and Protocols provides scientist with a comprehensive guide to many of the methods and techniques used for exon skipping, such as methods on how to discriminate 'real polymorphisms' from mutations that affect splicing. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical Exon Skipping: Methods and Protocols seeks to aid scientists in the continuing study of exon skipping. 452 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Lipases are the most applied enzymes in organic synthesis due to their broad substrate acceptance and because of the availability of the molecular, biochemical, themodynamical and solvent engineering tools, which allows the optimization of lipases and lipase-catalyzed reactions. On the other hand, phospholipases are emerging as useful enzymes in food and pharmaceutical industries. In Lipases and Phospholipases: Methods and Protocols, expert researchers in the field provide key techniques to investigate these essential enzymes. Focusing on fundamental issues, current and new applications as well as practical step-by-step protocols, and the extensive applications of lipases and the potential application of phospholipases and its inhibitors. Written in the highly successful Methods in Molecular Biology(TM) series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Thorough and intuitive, Lipases and Phospholipases: Methods and Protocols aids scientists in continuing to study lipases, phopholipases and related enzymes. 564 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Together with early theoretical work in population genetics, the debate on sources of genetic makeup initiated by proponents of the neutral theory made a solid contribution to the spectacular growth in statistical methodologies for molecular evolution. Evolutionary Genomics: Statistical and Computational Methods is intended to bring together the more recent developments in the statistical methodology and the challenges that followed as a result of rapidly improving sequencing technologies. Presented by top scientists from a variety of disciplines, the collection includes a wide spectrum of articles encompassing theoretical works and hands-on tutorials, as well as many reviews with key biological insight. Volume 2 begins with phylogenomics and continues with in-depth coverage of natural selection, recombination, and genomic innovation. The remaining chapters treat topics of more recent interest, including population genomics, -omics studies, and computational issues related to the handling of large-scale genomic data. Written in the highly successful Methods in Molecular Biology(TM) series format, this work provides the kind of advice on methodology and implementation that is crucial for getting ahead in genomic data analyses. Comprehensive and cutting-edge, Evolutionary Genomics: Statistical and Computational Methods is a treasure chest of state-of the-art methods to study genomic and omics data, certain to inspire both young and experienced readers to join the interdisciplinary field of evolutionary genomics. 572 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Studies on Men's Health and Fertility provides a comprehensive series of up-to-the-minute reviews addressing the role of oxidative stress in the aetiology of reproductive pathologies in the male. This volume represents by far the most detailed, authoritative review of the field that has been produced to date. The text encompasses the basic science of reactive oxygen species (ROS) production by mammalian spermatozoa, the way in which these highly reactive molecules are processed by the germ line and the physiological significance of this redox activity in the generation of a functional gamete. The factors responsible for perturbing the delicate balance between physiological redox signaling on the one hand and oxidative stress on the other are also extensively reviewed and some of the first clues concerning the underlying mechanisms (age, heat, infection, cryostorage, aberrant lipid metabolism), clearly identified. From a clinical perspective there are chapters setting out the methods we should be using to diagnose oxidative stress in the male germ line, a clinical perspective on the aetiology of this condition and detailed considerations of the most suitable means of ameliorating such stress from a therapeutic point of view. Studies on Men's Health and Fertility is intended to provide clinicians and scientists with a snap shot of the current status of this exciting, rapidly moving field. The book will be of value to clinicians interested in strategies for the management of oxidative stress in their infertility patients and scientists wishing to understand the molecular mechanisms underpinning the generation of ROS by these cells and its pathophysiological significance. It was not so long ago that the ability of spermatozoa to generate ROS was a hotly disputed topic. With the publication of this book such doubts can finally be laid to rest. There is now no doubt that these cells actively generate ROS, that oxidative stress is a major contributor to defects in male reproductive health and that the successful clinical management of this condition depends on developing a deeper understanding of the underlying molecular mechanisms. In this quest, Studies on Men's Health and Fertility will be seen as a clear and important milestone. 688 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Much work over the last two decades has firmly established that loss of cell cycle checkpoint regulation, and resultant unabated cellular proliferation, is an inherent characteristic of cancer. This loss may occur through aberration in any single component involved in signal transduction pathways that orchestrate checkpoint regulation, which may manifest through either a failure to activate the checkpoint or a failure to respond to the activated checkpoint. In normal cells, checkpoint pathways are activated when genetic or cellular homeostasis is compromised, and signals are then transduced to re-stabilize homeostasis, and, failing this, to activate the apoptotic machinery to induce a cellular suicidal response. This implies that both survival and cell death pathways are induced following checkpoint activation, and that the final decision is dependant on the net result of integrating the two sets of signals.It is intriguing that checkpoint pathways are also critical in cancer therapy to provide an apoptotic stimulus when cellular damage induced by the therapeutic agent is detected by the sensor system. Therefore, it is not surprising that failure in pro-survival checkpoint response will render tumor cells hypersensitive to cytotoxics and, conversely, failure in pro-apoptotic checkpoint response will induce genetic instability and/or therapeutic resistance. Understanding the intricacies of checkpoint response is, therefore, central to the design of therapeutic regimen that will enhance antitumor effects. Although early versions of this design entail combination of cytotoxic agents with cell cycle or checkpoint inhibitors, a greater understanding of the concepts could make such combinations clinically more effective. The contributions in this book will consolidate the current state of knowledge on checkpoint responses that may lay the foundation for hypothesis-driven rational approaches in advancing the management of cancer. The immediate attraction of the book to the scientific community is that it represents a timely opportunity to build upon existing concepts of checkpoints to expand our understanding of the inner workings of the critical checkpoint machinery. The present understanding has provided ample appreciation that response to checkpoint activation is manifested through coordinated inhibition of cyclin-dependent kinase (CDK) complexes in G1, S and/or the G2 phase in order to arrest the cell cycle. Kinase inhibition can occur through several mechanisms, including inhibitory phosphorylation of CDK, destruction of the cognate cyclins, and recruitment of CDK inhibitors from the INK and WAF1/CIP1 families. However, the wealth of information from recent discoveries needs to be examined critically to consolidate our conceptual knowledge of checkpoints. At the same time, there is acute awareness in the diversity of checkpoint response between cytotoxic agents, and this serves as a reminder of the magnitude of complexity that is inherent in checkpoint regulation. This volume is intended to bring the cancer research community closer toward an improved understanding of this regulation, how checkpoint abnormalities can impact negatively on cancer therapy, and emerging strategies to target checkpoint response as a therapeutic end-point. 288 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Heart failure affects over 5 million patients in the United States alone, and is a chronic and debilitating disease. While a number of pharmacologic therapies have shown varying degrees of effectiveness, many recent advances in the treatment of heart failure has focused on device based therapies. In Device Therapy in Heart Failure, William H. Maisel and a panel of authorities on the use and implementation of device based therapies provide a comprehensive overview of the current and developing technologies that are used to treat heart failure. Individual chapters provide an in-depth analysis of devices such as CRT's and ICD's, while broader topics such as the pathophysiology of heart failure and its current medical therapies are also discussed. Additional topics include Pacing and Defibrillation for Atrial Arrhythmias, Atrial Fibrillation Ablation, and Percutaneous Treatment of Coronary Artery Disease. 412 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Diabetes, particularly type 2, has become increasingly more common around the world. Consequently, the effect of diabetes on the brain has achieved enormous public health importance. A surge in pre-clinical and clinical research on topics ranging from management of hyperglycemia in acute stroke to disturbances in insulin signaling in Alzheimer's disease has led to substantial progress in the field. Written by a panel of international experts, Diabetes and the Brain provides in depth reviews on the cerebral complications of diabetes, and offers introductory chapters on current insights on the pathophysiology and clinical management of diabetes, as well as neuropsychological assessment and dementia. This relevant and easily accessible book explains the cerebral complications of diabetes, with an update on diabetes for neurologists, psychiatrists, and mental health providers and researchers in general,and on stroke and dementia for those involved in research and clinical practice in diabetes. 488 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Challenges for the treatment of valvular heart disease include the growing need for effective yet less invasive interventions and therapies to treat these progressive conditions. With the development of potential new treatments, it is crucial for cardiac physicians to be well informed on the pathophysiology, assessment, treatment options and their outcomes of valvular diseases. Written by a highly experienced and internationally recognized group of cardiologists, cardiac surgeons, and researchers, Valvular Heart Disease offers insights into the widely varying hemodynamic effects and clinical course of heart valve conditions, as well as the contemporary management of these conditions. Offering a broad perspective on these diseases, Valvular Heart Disease expands on the recent guidelines developed by the major heart societies in the United State and Europe. 548 pp. Englisch.