springer new york springer us nov 2018 (11 risultati)

Buch. Condizione: Neu. Neuware -This book describes current methods for the identification and characterization of the major hallmarks of senescent cells. Chapters focus on the high heterogeneity of the senescence phenotypes, and techniques to induce and identify specific senescence programs. Additional chapters describe cellular and mouse models in which is possible to study the complex cell and non-cell autonomous functions of senescent cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Cellular Senescence: Methods and Protocols aims to ensure successful results in the further study of this vital field.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 272 pp. Englisch.

Buch. Condizione: Neu. Neuware -This practical, hands-on volume examines the use of decellularized tissues and organs as biologically-active scaffolds by providing numerous approaches from experts in the field. While knowledge of how to grow and differentiate cells in culture has dramatically improved over time, the book addresses the challenges of how to instruct particular cells of interest to recognize and respond to their environment so as to proliferate, differentiate, and function for restoration of original tissue and organ form and function. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 364 pp. Englisch.

Buch. Condizione: Neu. Neuware -This detailed book provides a set of protocols necessary for the development of a variety of microchip-based electrophoretic assays. It compiles a range of such electrophoretic methods by leading researchers in the field, covering subjects such as microfluidic device fabrication, on-chip sample preparation, theoretical/simulation protocols for assessing these separation methods, as well as common practices followed when applying them to important real world applications. The contents of the book range from protocols for classical assays to those involving pioneering separation techniques recently developed by the scientific community for advancing our analytical capabilities. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 268 pp. Englisch.

Buch. Condizione: Neu. Neuware -Ethnobiology and ethnoecology have become very popular in recent years. Particularly in the last 20 years, many manuals of methods have published the most classical approaches to the subject. There have been, however, many advances in research as a result of interaction with different disciplines, but also due to more recent results, new original and interesting questions.This handbook provides the current state of the art methods and techniques in ethnobiology and ethnoecology, and related fields. This new volume, besides bringing new and original aspects of what is found in the literature, fills some of the gaps in volume one by including the most systematic and extensive treatment of methods and techniques in qualitative research. Along with the various methods covered in the individual chapters, the handbook also includes an extensive bibliography that details the current literature in the field.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 360 pp. Englisch.

Buch. Condizione: Neu. Neuware -This volume explores the latest techniques used to study tumor immunology. The chapters in this book detail methodologies for functional analysis and expansion of T lymphocytes for cancer research. The chapters also cover topics such as how single-cell RNA-sequencing can be exploited to dissect immune cell heterogeneity and precursors; isolating and expanding natural killer (NK) cells; evaluating NK cell-mediated anti-tumor killing activity in vitro; immunosurveillance orchestrated by specific immune subsets; use of HLA peptidomics for cancer-exome based identification of tumor neo-antigens; gold standard assessment of immunogenic cell death in oncological mouse models and methods to look at the therapeutic relevance of immune modulation in cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Cancer Immunosurveillance: Methods and Protocols is a valuable resource to aid researchers in better understanding and experimenting in this exciting and developing field.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 380 pp. Englisch.

Buch. Condizione: Neu. Neuware -This volume provides methods to study bone morphogenetic proteins (BMPs) and BMP modulators in cell culture, in both invertebrate and vertebrate animal models, and for therapeutic applications. Chapters guide the reader in the use of primary and immortalized cell lines, methodologies that exploit transcription and receptor mechanisms, newly developed animal models using nematodes, flies, amphioxus, frogs, zebrafish, and genetically engineered mice, and pre-clinical approaches to understanding BMP function in bone regeneration, heterotopic ossification, and cancers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Bone Morphogenetic Proteins: Methods and Protocols aims to ensure successful results in the further study of this vital field.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 280 pp. Englisch.

Buch. Condizione: Neu. Neuware -The serendipitously discovered link between developmental biology and cancer, touched of an explosion of discoveries on the role of Notch in human malignancies, including every aspect of cancer biology, from control of differentiation, proliferation and apoptosis in transformed cells to angiogenesis, tumor-stroma interaction and anti-cancer immune responses. A number of observations have revealed that Notch even plays a role in the renewal of cancer stem cells and tumor initiating cells, which are thought to be a major cause of resistance to treatment. Targeting Notch in Cancer will provide researchers, oncologists, pharmacologists and students with a detailed understanding of the intricate cross-talk between Notch and other pathways of therapeutic interest so to better design rational drug combinations for specific diseases and disease subsets.Divided into two parts, Part I describes in detail what we know about the genetics, molecular biology, biochemistry andstructural biology of Notch, as well as the role of Notch in such processes as angiogenesis and immune surveillance. Without insights gained from these basic studies, rational targeting of Notch in human disease would be impossible. Part II describes the role of Notch and ongoing experimental therapeutic efforts in the most important subtypes of human cancers, organized in a clinically oriented fashion by organs and systems affectedSpringer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 400 pp. Englisch.

Buch. Condizione: Neu. Neuware -This volume explores the latest advances in high-throughput phenotyping to screen milling quality, cooking and nutritional quality in breeding material, and simplifying grain quality screening in germplasm. The chapters in this book cover topics such as seed development stages; panicle architectural traits to understand yield components; starch structure and protein properties; and genome editing technologies to reveal grain quality and nutritional traits. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Rice Grain Quality: Methods and Protocols is a valuable resource for all researchers interested in expanding their knowledge in this evolving field.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 352 pp. Englisch.

Buch. Condizione: Neu. Neuware -This detailed volume explores barley as both a crop and a model, with practical techniques such as crossing barley, a range of tissue culture methods, the preparation of barley tissues for different forms of microscopy, and the assessment of sensitivity to abiotic stresses. Efficient protocols are provided for transformation, TILLING, virus-induced gene silencing and genome editing. There is also particular emphasis on a range of protocols for genotyping and for the analysis of gene expression. Written for the highly successful Methods in Molecular Biology series, chapters include introductions on their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Humana Press in Springer Science + Business Media, Heidelberger Platz 3, 14197 Berlin 328 pp. Englisch.

Buch. Condizione: Neu. Neuware -Fruits and fruit based products are, in most cases, associated with very good sensory characteristics, health, well-being, perishability, relatively easy to mix with food products of diverse origin, amenable to be processed by conventional and novel technologies. Given the multiplicity of aspects whenever fruit preservation is considered, the editors took the challenge of covering in a thorough, comprehensive manner most aspects dealing with this topic.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 548 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This book introduces the ade4 package for R which provides multivariate methods for the analysis of ecological data. It is implemented around the mathematical concept of the duality diagram, and provides a unified framework for multivariate analysis. The authors offer a detailed presentation of the theoretical framework of the duality diagram and also of its application to real-world ecological problems. These two goals may seem contradictory, as they concern two separate groups of scientists, namely statisticians and ecologists. However, statistical ecology has become a scientific discipline of its own, and the good use of multivariate data analysis methods by ecologists implies a fair knowledge of the mathematical properties of these methods.The organization of the book is based on ecological questions, but these questions correspond to particular classes of data analysis methods. The first chapters present both usual and multiway data analysis methods. Further chapters are dedicated for example to the analysis of spatial data, of phylogenetic structures, and of biodiversity patterns. One chapter deals with multivariate data analysis graphs.In each chapter, the basic mathematical definitions of the methods and the outputs of the R functions available in ade4 are detailed in two different boxes. The text of the book itself can be read independently from these boxes. Thus the book offers the opportunity to find information about the ecological situation from which a question raises alongside the mathematical properties of methods that can be applied to answer this question, as well as the details of software outputs. Each example and all the graphs in this book come with executable R code.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 344 pp. Englisch.