springer us nov 2020 (88 risultati)

Taschenbuch. Condizione: Neu. Neuware -Growing interest in the field of mental health in the workplace among policy makers, clinicians, and researchers alike has been fueled by equal employment rights legislation and increasing disability statistics in mental heath. The importance of addressing this topic is underscored by the fact that depression now ranks second on the hierarchy of occupational disabilities. The problem is compounded by a host of factors, including major difficulties in job retention and productivity experienced by persons with mental health disabilities; younger age and higher education of persons with mental health problems; and labor shortages and an aging workforce in many industrialized countries. In addition, particularly in the United States, the vocational needs of army veterans returning from duty with mental health disorders require system-based solutions and new rehabilitation approaches.The pressure created by these powerful legislative, societal, and economic forces has not been matched by the state of evidence-based practices in the field of employment retention and job accommodation in mental health. Current research evidence is fragmented, limited in scope, difficult to access, and adversely affected by the traditional divide between the fields of psychiatry and psychology on one hand and interdisciplinary employment research and practices on the other. As a result, policy makers, employers, disability compensation systems, and rehabilitation and disability management professionals have been left without a critical 'how to' evidence-informed toolbox for occupational practices to accommodate and retain persons with mental health disabilities in the workplace. Currently, no single source of knowledge and research evidence exists in the field that would guide best practices. Yet the need for workplace accommodations for persons with mental health disabilities has been growing and, based on epidemiological trends, is anticipated to grow even more in the future. These trends leave physicians, psychologists, occupational therapists, vocational rehabilitation professionals, disability managers, human resource professionals, and policy makers poorly prepared to face the challenge of integrating and maintaining persons with mental health disabilities in the workplace.The aim of the Handbook is to close the gap between the needs of the professionals and networks that work with or study persons with mental heath disorders in an employment context and the actual knowledge base in the field. The Handbook will be written in language that can easily be understood by readers representing a multitude of disciplines and research paradigms spanning the mental health, rehabilitation, and employment fields of inquiry. The Handbook will contribute an integration of the best quantitative and qualitative research in the field, together with experts¿ consensus, regarding effective work retention and accommodation strategies and practices in mental health.The book will consist of five major sections, divided into chapters written by recognized experts in these areas.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 508 pp. Englisch.

Taschenbuch. Condizione: Neu. Neuware -This volume provides comprehensive methods from expert scientists working in the Chimeric Antigen Receptor T Cell (CAR-T Cell) field. Chapters guide readers through the state-of-art of CAR-T cell technology, CAR design and vector production, CAR-T cell generation and manufacturing, CAR-T cell characterization, and quality control. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Chimeric Antigen Receptor T Cells: Development and Production aims to be useful in the production of CAR-T cells, especially for therapeutic purposes.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 288 pp. Englisch.

Taschenbuch. Condizione: Neu. Neuware -Neutrophils, the most abundant white cells in humans, serve as the primary cellular defense against infection. This volume provides a concise set of protocols for assessing basic neutrophil functions and investigating specialized areas in neutrophil biology. Each of the protocols is written by leading researchers in the field and includes hints for success, as well as guidance for troubleshooting. In the third edition of Neutrophils: Methods and Protocols, chapters retained from previous editions have been have been updated and include many new approaches. In addition, the Third Edition contains a number of new chapters that were not included in the first or second editions. Part I contains overviews of neutrophil biology, function, and disorders. Part II describes commonly used methods to isolate neutrophils from humans and other animal species. Part III details methods for investigating chemotaxis, transmigration, phagocytosis, and bactericidal activity. Part IV includes protocols that measure neutrophil apoptosis, calcium signal transduction, degranulation and detection of cytoplasmic granules, gene expression, transcription factors, and apoptosis. Part V provides multiple assays for measuring production of intracellular and/or extracellular reactive oxygen species, and a method that details use of the cell-free NADPH oxidase assay, an iconic assay for studies of the neutrophil NADPH oxidase. Part VI provides chapters that describe how to analyze formation and function of neutrophil extracellular traps. Neutrophil Methods and Protocols: Third Edition is a comprehensive source for detailed explanations and applications of the most modern methodological advances in neutrophil biology. Both basic scientists and clinicians will find a collection of this caliber to be an invaluable aid in their work with neutrophils.Features-Provides neutrophil protocols that are broadly applicable to leukocyte biology-Includes protocols for isolation of neutrophils from humans and animal species-Presents an interesting and informative overview of the field of neutrophil biology-Facilitates a better understanding of the role of neutrophils in host defenseReadershipMolecular and Cell Biologists, Immunologists, Infectious Disease Specialists, Microbiologists 488 pp. Englisch.

Taschenbuch. Condizione: Neu. Neuware -This book is a collection of principles and current practices in omics research, applied to skeletal muscle physiology and disorders. The various sections are categorized according to the level of biological organization, namely, genomics (DNA), transcriptomics (RNA), proteomics (protein), and metabolomics (metabolite). With skeletal muscle as the unifying theme, and featuring contributions from leading experts in this traditional field of research, it highlights the importance of skeletal muscle tissue in human development, health and successful ageing. It also discusses other fascinating topics like developmental biology, muscular dystrophies, exercise, insulin resistance and atrophy due to disuse, ageing or other muscle diseases, conveying the vast opportunities for generating new hypotheses as well as testing existing hypotheses by combining high-throughput techniques with proper experiment designs, bioinformatics and statistical analyses.Presenting the latest researchtechniques, this book is a valuable resource for the physiology community, particularly researchers and grad students who want to explore the new opportunities for omics technologies in basic physiology research.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 228 pp. Englisch.

Buch. Condizione: Neu. Neuware -The third edition of Health Services Planning represents a necessary revision of the second edition (2003) and reflects the significant changes that have occurred in the social and health environments, the healthcare field, and the planning endeavor itself. The book reviews the history of health planning (with reference to activities in other countries) before describing the process from start to finish in great detail. The philosophy underlying health planning and its perception, past and present, are reviewed with a particular emphasis on the factors that are making health planning more relevant than ever.The steps in the health services planning process are reviewed and supported by how-to guidance, examples of planning applications, and planning case studies. The various levels and types of planning are described (societal, community, organizational; strategic, business, marketing), and the similarities and differences discussed.Newand updated chapters in this edition cover the planning requirements introduced through the Affordable Care Act, the rethinking of the community health needs assessment process, and the implications of the population health movement for health planning:The Social and Health Systems Context for Health Services PlanningThe Changing Environment for Health PlanningThe New Community Assessment ProcessFrom Community Health Needs Assessment to Population Health AssessmentHealth Services Planning, 3rd Ed., remains a critical resource for health administration programs, as well as an essential publication for health professionals in both public and private sector healthcare organizations that need to have an understanding of the planning process.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 484 pp. Englisch.

Buch. Condizione: Neu. Neuware -Providing a comprehensive presentation of the diagnosis, evaluation and management of malunions, this generously illustrated text details the current principles, surgical techniques and approaches with these challenging clinical situations. Since each malunion can be fairly specific, the treatment regimens provide guidelines to effectively approach such problems. Opening with a brief overview of the principles of malunions, the remainder of the book is divided by anatomical area and provides evidence-based recommendations, case examples, and preferred treatment/algorithms for both the upper and lower extremities, including the clavicle, proximal and distal humerus, hand and wrist, proximal and distal femur, and tibia and ankle, as well as the pelvis and acetabulum. Specialized circumstances are also discussed, including periprosthetics and joint replacement.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 420 pp. Englisch.

Taschenbuch. Condizione: Neu. Neuware -Rapid developments in brain neuroimaging methods have occurred over the past decade. These advances have revolutionized cognitive and behavioral neuroscience, and are likely to have major influence on clinical psychological, psychiatric, and neurological practice over the coming years. There are a number of excellent books that focus on specific neuroimaging methods, such as fMRI. Furthermore, cognitive and neuroscience texts have increasingly incorporated functional brain neuroimaging. Yet, there are few books to date that consider and review emerging research in the application of brain neuroimaging methods for the study and assessment of behavioral and cognitive disorders. This book provides a broad coverage of current research trends in the clinical application of brain neuroimaging methods in the context of behavioral medicine, neuropsychology, and related areas of medical psychology. It uniquely integrates current neuroimaging methods and studies with current behavioral medicineresearch, and presents knowledge derived from recent developments in the fields of functional and structural brain imaging. By integrating information from experimental behavioral medicine with clinical insights, this book will serve as a source book for neuropsychologists, psychologists, neurologists, psychiatrists, and other professionals in both clinical practice and academic context. This integration results in the reader having a greater understanding of how the brain controls behavior, the disturbances of behavior that may occur with different disorders, and what clinicians should consider when assessing or working with patients with behavioral problems.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 420 pp. Englisch.

Taschenbuch. Condizione: Neu. Neuware -This volume describes modern approaches for the detection and analysis of inositol phosphates from various biological, clinical, and environmental samples. Chapters focus on methods to study enzymes that modify inositol signals, chemical biology of inositol phosphates, and methods to synthesize and purify inositol phosphates and their analogs. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Inositol Phosphates: Methods and Protocol aims to ensure successful results in the further study of this vital field.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 196 pp. Englisch.

Taschenbuch. Condizione: Neu. Neuware -This volume covers a diverse collection of protocols currently being used by the Toxoplasma research community, and also looks at innovative methods that are pushing the boundaries of possibilities. Chapters in this book discuss topics such as isolation and genotyping of Toxoplasma gondii strains; assessing rhoptry secretion in T. gondii; plate-based quantification of stimulated Toxoplasma egress; methods to study ocular toxoplasmosis; and metabolic analysis of Toxoplasma gondii tachyzoites. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and thorough, Toxoplasma gondii: Methods and Protocols is a valuable resource for students or groups starting off in the field, as well as laboratories interested in implementing the latest techniques described in the book.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 484 pp. Englisch.

Taschenbuch. Condizione: Neu. Neuware -This volume covers the latest techniques and strategies used in multi-photon excitation (MPE) microscopy. Chapters in this book cover the fundamentals of MPE microscopy as applied to both in vitro and in vivo experimental systems; information on how to combine MPE microscopy with targeted electrophysiological recordings, calcium imaging, and transmembrane voltage imaging; methods to investigate cellular and large-scale neural morphology; signaling in astrocytes; and ways to use MPE microscopy to study the retina. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 376 pp. Englisch.

Taschenbuch. Condizione: Neu. Neuware -This volume provides basic and advanced protocols on somatic cell nuclear transfer, induced pluripotent stem cells, and direct reprogramming of somatic cells into different functional cells. Chapters guide readers through methods on standardized procedures for characterization of induced pluripotent stem cells, as well as those for preparation of materials required for induction of pluripotent stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Nuclear Reprogramming: Methods and Protocols aims to ensure successful results in the further study of this vital field.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 320 pp. Englisch.

Taschenbuch. Condizione: Neu. Neuware -This volume looks at--and discusses the techniques of--a range of areas in biology in which macrophage migration inhibitory factor (MIF) is studied. Topics covered in this book include methods to quantify MIF in cell supernatants and human samples, using ELISA and western blot; detection and analysis of intracellular MIF using flow cytometry; genotyping of human MIF polymorphisms, detection of MIF expression in a marine invertebrate using rapid amplification of cDNA RACE; studying the MIF receptor CD74 in tissues using immunohistochemistry; and studying MIF in models of disease such as asthma, Plasmodium infection, and gout. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and practical, Macrophage Migration Inhibitory Factor: Methods and Protocols is a valuable resource that will help researchers gain a new understanding of MIF biology in health and disease.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 280 pp. Englisch.

Taschenbuch. Condizione: Neu. Neuware -This volume provides detailed protocols and methodologies required to perform bioluminescent imaging in multiple stages, enabling the reader to integrate this technology into their laboratory-based imaging experiments. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Bioluminescent Imaging: Methods and Protocols to ensure successful results in the further study of this vital field.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 260 pp. Englisch.

Taschenbuch. Condizione: Neu. Neuware -This volume provides protocols for mouse models of allergic diseases and guidelines for choosing a particular strains, allergen, adjuvant, and route of sensitization. Chapters detail types of allergic disease, methods that are frequently employed to analyze pathophysiology of allergic diseases, manipulation of intestinal microbiota, and desensitization of immure responses in animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Animal Models of Allergic Disease: Methods and Protocols aims to offer a comprehensive collection of protocols and experience-derived instructions to further allergic disease research.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 376 pp. Englisch.

Taschenbuch. Condizione: Neu. Neuware -This volume explores detailed methods and experimental protocols evaluating the effect of a compound or a mixture of compounds on the action of enzymes that are significant targets in pharmaceuticals. Consisting of three sections, the book delves into recent biocomputing and bioinformatics protocols, state-of-the art modern biophysical, electrophoretic, and chromatographic methods and high-throughput screening approaches, as well as detailed protocols and examples of the inhibition analysis and evaluation of selected enzymes. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 304 pp. Englisch.

Taschenbuch. Condizione: Neu. Neuware -Neutrophils, the most abundant white cells in humans, serve as the primary cellular defense against infection. This volume provides a concise set of protocols for assessing basic neutrophil functions and investigating specialized areas in neutrophil biology. Each of the protocols is written by leading researchers in the field and includes hints for success, as well as guidance for troubleshooting. In the third edition of Neutrophils: Methods and Protocols, chapters retained from previous editions have been have been updated and include many new approaches. In addition, the Third Edition contains a number of new chapters that were not included in the first or second editions. Part I contains overviews of neutrophil biology, function, and disorders. Part II describes commonly used methods to isolate neutrophils from humans and other animal species. Part III details methods for investigating chemotaxis, transmigration, phagocytosis, and bactericidal activity. Part IV includes protocols that measure neutrophil apoptosis, calcium signal transduction, degranulation and detection of cytoplasmic granules, gene expression, transcription factors, and apoptosis. Part V provides multiple assays for measuring production of intracellular and/or extracellular reactive oxygen species, and a method that details use of the cell-free NADPH oxidase assay, an iconic assay for studies of the neutrophil NADPH oxidase. Part VI provides chapters that describe how to analyze formation and function of neutrophil extracellular traps. Neutrophil Methods and Protocols: Third Edition is a comprehensive source for detailed explanations and applications of the most modern methodological advances in neutrophil biology. Both basic scientists and clinicians will find a collection of this caliber to be an invaluable aid in their work with neutrophils.Features¿ Provides neutrophil protocols that are broadly applicable to leukocyte biology¿ Includes protocols for isolation of neutrophils from humans and animal species¿ Presents an interesting and informative overview of the field of neutrophil biology¿ Facilitates a better understanding of the role of neutrophils in host defenseReadershipMolecular and Cell Biologists, Immunologists, Infectious Disease Specialists, MicrobiologistsSpringer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 488 pp. Englisch.

Taschenbuch. Condizione: Neu. Neuware Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 348 pp. Englisch.

Taschenbuch. Condizione: Neu. Neuware -This book focuses on ion mobility-mass spectrometry (IM-MS) and informatics approaches to improve traditional analysis of molecules by providing fundamentals and protocols for exploiting the potential of state-of-the-art IM-MS technology for the most common analytical applications. The chapters have been organized into four parts, each dealing with a particular set of IM-MS applications: 1) metabolomics and lipidomics; 2) proteomics and glycomics; 3) imaging and ambient ionization IM-MS; and 4) bioinformatic solutions for analyzing IM-MS data and deriving CCS values. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 324 pp. Englisch.

Buch. Condizione: Neu. Neuware -This volume provides basic and advanced protocols on somatic cell nuclear transfer, induced pluripotent stem cells, and direct reprogramming of somatic cells into different functional cells. Chapters guide readers through methods on standardized procedures for characterization of induced pluripotent stem cells, as well as those for preparation of materials required for induction of pluripotent stem cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Nuclear Reprogramming: Methods and Protocols aims to ensure successful results in the further study of this vital field.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 320 pp. Englisch.

Buch. Condizione: Neu. Neuware -This detailed book collects standard techniques and cutting-edge methods that are frequently used by the research community studying the fungus Trichoderma reesei. Beginning with overviews of its evolution, its use in the production of industrially-relevant enzymes, and synthetic biology applications, the volume continues with methods covering topics from transformation techniques and gene editing to downstream-analytical applications and -omics analyses and the corresponding bioinformatics approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 376 pp. Englisch.

Buch. Condizione: Neu. Neuware -This volume provides the most cutting edge technologies related to the study of integrin activation and the characterization of their vast interactomes. Chapters detail protocols on experimental approached to quantify focal adhesion parameters, integrin activation, and the lateral interaction of integrins with transmembrane binding partners. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, The Integrin Interactome: Methods and Protocols aims to give the reader a multi-scale journey from single bonds inside protein structures to the function of these crucial adhesion receptors at a whole organism level in physiology and pathology.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 336 pp. Englisch.

Buch. Condizione: Neu. Neuware -This volume explores major light microscopic imaging modalities that can be used to view nervous tissue, and discusses the steps needed to use each of them, and ways to interpret the data. The chapters in this book cover topics such as atlasing of insect brain; neuroanatomical tracing through fluorochrome expression; fluorescent probes for amyloids; or optical clearing for ultramicroscopy of GFP-expressing tissues. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 488 pp. Englisch.

Taschenbuch. Condizione: Neu. Neuware -This volume details key protocols for developing strategies in immunotherapy. Chapters guide the readers through protocols related to various DNA, RNA and protein methods to reprogram the immune system, immune cells, analyzing the effect of the reprogrammed cells, and key methods to consider and analyze patients enrolled in clinical trials with novel immunotherapy regimens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Cell Reprogramming for Immunotherapy: Methods and Protocols aims to ensure successful results in the further study of this vital field.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 356 pp. Englisch.

Buch. Condizione: Neu. Neuware -This second edition provides a comprehensive laboratory manual on skeletal development and skeletal repair research utilizing mouse models. Chapters provide methods and protocols on the most current and cutting-edge techniques in skeletal development and repair, histological, cellular, and molecular techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Skeletal Development and Repair: Methods and Protocols-Second Edition aims to ensure successful results in the further study of this vital field.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 500 pp. Englisch.

Buch. Condizione: Neu. Neuware -This volume provides protocols for mouse models of allergic diseases and guidelines for choosing a particular strains, allergen, adjuvant, and route of sensitization. Chapters detail types of allergic disease, methods that are frequently employed to analyze pathophysiology of allergic diseases, manipulation of intestinal microbiota, and desensitization of immure responses in animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Animal Models of Allergic Disease: Methods and Protocols aims to offer a comprehensive collection of protocols and experience-derived instructions to further allergic disease research.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 376 pp. Englisch.

Buch. Condizione: Neu. Neuware -This volume introduces engineers and healthcare professionals to the latest in neurosurgical robotic technology. The chapters in this book are organized into two parts and cover basic engineering concepts that underpin surgical robotics; various robotic platforms and how these systems make their way to the clinic; popular applications of surgical robots in neurosurgery within subspecialties; and a discussion on the future development of neurosurgical robotic systems. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your clinic.Cutting-edge and thorough, Neurosurgical Robotics is a valuable resources for scientists and engineers interested in learning more about this fascinating and developing field.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 244 pp. Englisch.

Buch. Condizione: Neu. Neuware -This second edition focuses on various techniques to investigate aspects of the TNF Superfamily members in health and disease. Chapters detail protocols on the signaling process of TNF family members, technical examples to investigating the role of TNF family members in physiopathologies, modulation of TNF signaling by pathogens, experimental applications of TNF-reporter mice, methodologies for various assays of TNF family members and the production of recombinant molecules. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and accessible, The TNF Superfamily: Methods and Applications, Second Edition serves to aid researchers investigating this key family of proteins.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 300 pp. Englisch.

Buch. Condizione: Neu. Neuware -This volume provides basic and cutting-edge methods and protocols to study the major characteristics of eukaryotic cells. Chapters detail the different pathways of endocytosis in vivo, real time imaging of endocytic steps, endocytosis in model organisms, super-resolution methods to follow proteins involved in exocytosis, specific protocols for exocytosis in specialized cells such as neutrophils or neuroendocrine cells, as well as secretion of exosomes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and clearly written, Exocytosis and Endocytosis: Methods and Protocols is a valuable resource for researchers in the fields of cell biology, neurology, immunology, oncology, and those interested in studying protein trafficking and signal regulation.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 344 pp. Englisch.

Buch. Condizione: Neu. Neuware -This volume explores various experimental approaches that can be used to investigate myeloid-derived suppressor cells (MDSC) and can be useful to understand the characterization of these cells. The chapters in this book cover topics such as the study of MDSC in human patient samples; employing flow cytometry or magnetic enrichment; immunophenotyping using multi-parameter flow cytometry and mass cytometry; functional characterization of MDSC by biochemical, immunological, and microscopy approaches; MDSC characterization through the use of high-resolution genomics; and novel methods of high-resolution visualization of living tissue and the purification of granulocytes. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 232 pp. Englisch.

Buch. Condizione: Neu. Neuware -This volume provides the most current methods to study RNA remodeling proteins. Chapters detail methods, ranging from basic to complex, procedures to identify RNA remodeling proteins and their cofactors, physiological RNA targets and biological functions, and complex molecular mechanisms of action using purified components. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, RNA Remodeling Proteins: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 452 pp. Englisch.