springer us springer us jun 2024 (19 risultati)

Buch. Condizione: Neu. Neuware -This fully updated new edition explores new techniques for studying plant stress. This includes novel methodologies such as MeRIP-seq for identifying changes in m6A profiles, isolation of stress granules, and additional methodologies such as MNase¿seq for identifying nucleosome occupancy, alternative splicing analysis, identifying proteins that interact with long noncoding RNAs, untargeted metabolomics, ROS and NO measurements, priming-related protocols, growth-promoting bacteria isolation and functional characterization, as well as isolating mutants for stress-regulated genes using CRISPR technology. Written for the highly successful Methods in Molecular Biology series, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Plant Stress Tolerance: Methods and Protocols, Third Edition provides a wide range of protocols catering to the needs of plant physiologists, biochemists, and molecular biologists interested in probing this vital area of study.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 308 pp. Englisch.

Buch. Condizione: Neu. Neuware -This volume provides references for methods about the proteomics of microbial communities, also called metaproteomics. Chapters guide readers first through specific protein extractions from different environments and/or ecological niches crowded by heterogeneous microbial communities, then deepening the possible complete metaproteomic workflows in several situations or conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Metaproteomics: Methods and Protocols aims to ensure successful results in the further study of this vital field.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 236 pp. Englisch.

Buch. Condizione: Neu. Neuware -This detailed volume presents cutting-edge approaches to study infection, immunity, and intervention strategies against intracellular pathogens. The book explores areas such as how intracellular pathogens infect and survive inside the phagocytes, immunopathological features of infection, evasion of host-induced immune responses, cellular crosstalk in the presence of intracellular pathogens, host innate and adaptive immunity, and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 388 pp. Englisch.

Buch. Condizione: Neu. Neuware -This edition provides a collection of state-of-art methods and tools for human leukocyte antigen (HLA) and major histocompatibility complex (MHC) research. The book explores updated as well as novel in silico tools, resources, and wet lab protocols for HLA typing, including determination of the HLA class I and class II type of an individual in clinical work and research, such as in transplantation medicine and vaccine development in the context of infectious diseases or cancer immunotherapies. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detailed information and implementation advice that leads to best results.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 312 pp. Englisch.

Buch. Condizione: Neu. Neuware Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 304 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume explores the latest advancements made in the field of mass spectrometry (MS) based single cell proteomics. The chapters in this book focus on technologies, methods, and biological applications and together cover the entire single cell proteomics analysis workflow, from sample collection and preparation through liquid chromatographic separation and mass spectrometric data acquisition to data analysis and interpretation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and authoritative, Mass Spectrometry Based Single Cell Proteomics: Methods and Protocols is a valuable resource for all researchers interested in using these technologies and methods in their laboratories to continue making biological discoveries in this important field. 256 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed volume explores the frontiers of this new era in cancer cytogenetics and cytogenomics, focusing on establishing a karyotype as an information-based genomic framework, as well as presenting technological platforms for collecting and analyzing data at the genome level. It begins with several conceptual chapters that introduce ideas such as the Genome Architecture Theory, forcefully emphasizing the importance of cytogenomics in the post-genomics era. The book then proceeds with protocols covering both basic and advanced cytogenetic and cytogenomic methods, as well as diverse experiments beyond traditional cytogenetic platforms, and bioinformatics techniques and resources. Written for the highly successful Methods in Molecular Biologyseries, chapters include introductionsto their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Cancer Cytogenetics and Cytogenomics: Methods and Protocolsserves as an ideal guide to the unique power of this area of research in spatial biology and system-integrated genomics. 412 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This volume covers the latest translational research methods used to study Alcohol Use Disorders (AUD). The chapters in this book highlight cross-species complementary approaches and discusses ways that this translational work will further advance scientific progress in this field. In the Neuromethods series style, chapter include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Cutting-edge and practical, Translational Research Methods for Alcohol Use Disorders is a valuable resource for all students and researchers interested in expanding their knowledge of AUD.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 216 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides methods to analyze RNA and gain insight to a number of critical cellular functions. Chapters detail RNA isolation and enrichment, RNA amplification and detection, RNA sequencing, analysis of RNA modification, RNA delivery approaches, and analysis of RNA binding and catalytic activity. Written in the highly successfulMethods in Molecular Biologyseries format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge,RNA Amplifications and Analysis: Methods and Protocolsaims to present researchers withcutting-edge technologies with detailed explanations of critical steps, while providing a clear understanding of the overall protocol. 492 pp. Englisch.

Taschenbuch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This detailed volume presents a diverse set of methodological approaches designed to improve our understanding of bacterial infections from a wide range of bacterial species. Beginning with biofilms and subcellular compartments, the book explores transcriptional analysis, methods for studying plasmid dynamics, and tools for phylogenetic analysis of bacterial genomes, as well as bacterial effector proteins interfering with host systems, host response analysis, and in vivo and in vitro infection models. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 352 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides references for methods about the proteomics of microbial communities, also called metaproteomics. Chapters guide readers first through specific protein extractions from different environments and/or ecological niches crowded by heterogeneous microbial communities, then deepening the possible complete metaproteomic workflows in several situations or conditions. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Metaproteomics: Methods and Protocols aims to ensure successful results in the further study of this vital field. 236 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This fully updated new edition explores new techniques for studying plant stress. This includes novel methodologies such as MeRIP-seq for identifying changes in m6A profiles, isolation of stress granules, and additional methodologies such as MNase-seq for identifying nucleosome occupancy, alternative splicing analysis, identifying proteins that interact with long noncoding RNAs, untargeted metabolomics, ROS and NO measurements, priming-related protocols, growth-promoting bacteria isolation and functional characterization, as well as isolating mutants for stress-regulated genes using CRISPR technology. Written for the highly successful Methods in Molecular Biology series, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Plant Stress Tolerance: Methods and Protocols, Third Edition provides a wide range of protocols catering to the needs of plant physiologists, biochemists, and molecular biologists interested in probing this vital area of study. 308 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volume provides protocols on different combinations of contaminates, matrices, and sample preparation. Chapters are divided into two parts, detailing polycyclic aromatic hydrocarbon,dioxins, furans, organochlorie pesticides, toxic elements, mycotoxins, mercury in food products, acrylamine, polypeptide antibiotics, tetracyclines, coccidiostats, beta-blockers, sedatives,glucocorticoids, palytoxin-like marine biotoxins in fish, polar drugs and contaminants in animal feed, UV filters, micro and nanoplastics in seafood, tetracyclines in vegetables, MCPDEs, and pharmaceuticals in seafood. Written in the format of the Methods and Protocols in Food Science (MeFS) series, the chapters include an introduction to the respective topic, list necessary materials and reagents, detail well-established and validated methods for readilyreproducible laboratory protocols, and contain notes on how to avoid orsolve typical problems.Authoritative and cutting-edge,Chemical Food Contaminants Analysis aims to ensure successful results in the further study of this vital field 336 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This detailed volume explores the frontiers of this new era in cancer cytogenetics and cytogenomics, focusing on establishing a karyotype as an information-based genomic framework, as well as presenting technological platforms for collecting and analyzing data at the genome level. It begins with several conceptual chapters that introduce ideas such as the Genome Architecture Theory, forcefully emphasizing the importance of cytogenomics in the post-genomics era. The book then proceeds with protocols covering both basic and advanced cytogenetic and cytogenomic methods, as well as diverse experiments beyond traditional cytogenetic platforms, and bioinformatics techniques and resources. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and up-to-date, Cancer Cytogenetics and Cytogenomics: Methods and Protocols serves as an ideal guide to the unique power of this area of research in spatial biology and system-integrated genomics.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 412 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This volume explores the latest advancements made in the field of mass spectrometry (MS) based single cell proteomics. The chapters in this book focus on technologies, methods, and biological applications and together cover the entire single cell proteomics analysis workflow, from sample collection and preparation through liquid chromatographic separation and mass spectrometric data acquisition to data analysis and interpretation. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and authoritative, Mass Spectrometry Based Single Cell Proteomics: Methods and Protocols is a valuable resource for all researchers interested in using these technologies and methods in their laboratories to continue making biological discoveries in this important field.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 256 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This volume provides methods to analyze RNA and gain insight to a number of critical cellular functions. Chapters detail RNA isolation and enrichment, RNA amplification and detection, RNA sequencing, analysis of RNA modification, RNA delivery approaches, and analysis of RNA binding and catalytic activity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, RNA Amplifications and Analysis: Methods and Protocols aims to present researchers with cutting-edge technologies with detailed explanations of critical steps, while providing a clear understanding of the overall protocol.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 492 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This fully updated volume explores notable developments in the field of mammalian cell-based recombinant protein production. Beginning with methods for transient recombinant protein production, the book continues with methods for stable cell pool generation, protein production using stable clonal cell lines, as well as high-throughput screening technologies for characterizing transient cell surface protein ectodomain expression and for identifying host genes involved in protein production. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Recombinant Protein Expression in Mammalian Cells: Methods and Protocols, Second Edition serves as an ideal guide for researchers investigatingprotein structure and function and accelerating the discovery of new therapeutic proteins. 372 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This edition provides a collection of state-of-art methods and tools for human leukocyte antigen (HLA) and major histocompatibility complex (MHC) research. The book explores updated as well as novel in silico tools, resources, and wet lab protocols for HLA typing, including determination of the HLA class I and class II type of an individual in clinical work and research, such as in transplantation medicine and vaccine development in the context of infectious diseases or cancer immunotherapies. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detailed information and implementation advice that leads to best results.Up-to-date and practical, HLA Typing: Methods and Protocols, Second Edition serves as a valuable resource for any researcher interested in learning more about this vital field. 312 pp. Englisch.

Buch. Condizione: Neu. This item is printed on demand - Print on Demand Titel. Neuware -This fully updated volume explores notable developments in the field of mammalian cell-based recombinant protein production. Beginning with methods for transient recombinant protein production, the book continues with methods for stable cell pool generation, protein production using stable clonal cell lines, as well as high-throughput screening technologies for characterizing transient cell surface protein ectodomain expression and for identifying host genes involved in protein production. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Springer-Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg 372 pp. Englisch.