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Riassunto: It is now more than ten years since Dr. Alec Jeffreys (now Professor Sir Alec Jeffreys, FRS) reported in Nature that the investigation of certain minisatellite regions in the human genome could produce what he termed DNA fingerprints and provide useful information in the fields of paternity testing and forensic analysis. Since that time we have witnessed a revolution in the field of forensic identification. A total change of technology, from serological or electrophoretic analysis of protein polymorphisms to direct investigation of the underlying DNA polymorphisms has occurred in a short space of time. In addition, the evolution and development of the DNA systems themselves has been rapid and spectacular. In the last decade we have progressed from the multilocus DNA fing- prints, through single locus systems based on the same Southern blot RFLP technology, to a host of systems based on the PCR technique. These include Allele Specific Oligonucleotide (ASO)-primed systems detected by dot blots, the “binary” genotypes produced by mapping variations within VNTR repeats demonstrated by minisatellite variant repeat (MVR) analysis, and yet other fragment-length polymorphisms in the form of Short Tandem Repeat (STR) loci. Hand in hand with the increasing range of systems available has been the development of new instrumentation to facilitate their analysis and allow us to explore the possibilities of high volume testing in the form of mass scre- ing and offender databases.
From the Back Cover:
In Forensic DNA Profiling Protocols, leading forensic experts from around the world describe in detail their time-proven methods for identity testing through DNA analysis. Their state-of-the-art collection of easily reproducible methods includes all of the major techniques of DNA analysis currently used in forensic identity testing. The book covers PCR-based test systems, the now widely used STR typing systems (complete with details of both manual and automated detection systems), repeat unit mapping (MVR), direct-phase minisequencing, capillary electrophoresis, and direct blotting. Also fully discussed are the recovery of DNA from a wide range of sample types (blood, semen, skeletal remains, and saliva), species testing, sex determination, and mitochondrial DNA testing.
Forensic DNA Profiling Protocols captures and makes practical for all laboratories the whole array of new technologies and techniques that have revolutionized forensic science. The readily reproducible methods elucidated here represent the state-of-the-art for those scientists who want to introduce, establish, and perfect DNA identification techniques in their laboratories.
Titolo: Forensic DNA Profiling Protocols
Casa editrice: Springer Verlag
Data di pubblicazione: 1998
Condizione libro: New
Descrizione libro Humana Press, 1998. Condizione libro: Very Good. 1st. Ships from the UK. Former Library book. Great condition for a used book! Minimal wear. Codice libro della libreria GRP89914102
Descrizione libro Humana Press. Hardcover. Condizione libro: Good. Book shows minor use. Cover and Binding have minimal wear and the pages have only minimal creases. Codice libro della libreria G0896034437I3N00
Descrizione libro Humana Press. Hardcover. Condizione libro: Very Good. Book has appearance of light use with no easily noticeable wear. Codice libro della libreria G0896034437I4N00
Descrizione libro Humana Press, 1998. Hardcover. Condizione libro: Acceptable. Ex-library Satisfaction 100% guaranteed. Codice libro della libreria mon0001720605
Descrizione libro Humana Press. Condizione libro: Good. Ex-library, so some stamps and wear, and may have sticker on cover, but in good overall condition. Codice libro della libreria Z1-C-067-00304
Descrizione libro Humana Press. Condizione libro: Acceptable. Used - Acceptable. Sticker on cover. Ex-library with wear - may contain significant amounts of highlighting and underlining in pen or pencil. Codice libro della libreria Z1-C-067-00357
Descrizione libro Humana Press 1998-01-22, 1998. Hardcover. Condizione libro: good. 1998. 0896034437. Codice libro della libreria 713455
Descrizione libro Humana Press, 1998. Hardcover. Condizione libro: Used: Good. Codice libro della libreria 14863978
Descrizione libro Humana Press, 1998. Hardcover. Condizione libro: Used: Good. Ex-library. Normal library markings. Covers have light rubbing and wear. Corners and edges lightly rubbed. Clean text. Codice libro della libreria 141021017
Descrizione libro Condizione libro: Good. Forensic DNA Profiling Protocols (Methods in Molecular Biology). Codice libro della libreria New0128616