Biotechnology: DNA to Protein -- A Laboratory Project in Molecular Biology - Brossura

Thiel, Teresa; Bissen, Shirley T.; Lyons, Eilene M.

 
9780072416640: Biotechnology: DNA to Protein -- A Laboratory Project in Molecular Biology
Brossura
ISBN 10: 0072416645 ISBN 13: 9780072416640
Casa editrice: McGraw-Hill Higher Education, 2001

Sinossi

This one-semester, project-based laboratory manual gives junior/senior level students the opportunity to characterize the enzyme alpha-amylase. As students proceed through the sequenced experiments, they will learn the principles of DNA, RNA, and protein structure by using modern-day laboratory techniques. Genetics, cell biology, and organic chemistry are prerequisites.

Le informazioni nella sezione "Riassunto" possono far riferimento a edizioni diverse di questo titolo.

Contenuti

Examine Enzyme Activity of alpha-Amylase

1 Starch Plate Assay

2 Quantitative Enzyme Assay

3 Factors Affecting Enzyme Function

Examine alpha-Amylase Proteins

4 Analysis of Protein Structure Using RasMol

5 Analysis of alpha-Amylase Proteins

5A SDS-PAGE

5B Western Blotting

Examine DNA Structure

6 Analysis of DNA Structure Using RasMol

7 Isolation of Chromosomal DNA from Bacillus licheniformis

Find the alpha-Amylase Gene

8 PCR Amplification and Labeling of Probe DNA

9 Southern Hybridization

9A Restriction Enzyme Cleavage of Chromosomal DNA

9B Denaturation and Transfer of DNA to a Membrane

9C Southern Hybridization and Detection

Clone the alpha-Amylase Gene

10 Cloning the alpha-Amylase Gene

10A Cleavage of Chromosomal DNA

10B Cleavage of Plasmid DNA

10C Ligation of Chromosomal and Plasmid DNA

10D Transformation

10E Identification of alpha-Amylase Clones

Analyze alpha-Amylase Clones

11 Verification and Mapping of alpha-Amylase Clones

11A Verification of alpha-Amylase Clones Using PCR

11B Isolation of Plasmid DNA from alpha-Amylase Clones

11C Restriction Cleavage and Mapping of alpha-Amylase Plasmid DNA

11D Preservation of Recombinant Strains

11E Southern Analysis of alpha-Amylase Plasmid DNA

12 Enzyme Activity of alpha-Amylase Clones

Product Description

Book by Thiel Teresa Bissen Shirley T Lyons Eilene M

Le informazioni nella sezione "Su questo libro" possono far riferimento a edizioni diverse di questo titolo.

Editore
McGraw-Hill Higher Education
Data di pubblicazione
2001
Lingua
Inglese
ISBN 10 
0072416645
ISBN 13 
9780072416640
Rilegatura
Rilegatura a spirale
Numero di pagine
192
Contatto del produttore
non disponibile
Persona responsabile
non disponibile

Altre edizioni note dello stesso titolo

9780071122795: Biotechnology: DNA to Protein -- A Laboratory Project in Molecular Biology

Edizione in evidenza

ISBN 10:  0071122796 ISBN 13:  9780071122795
Casa editrice: McGraw-Hill Higher Education, 2001
Brossura