The emphasis of this lab manual is on the basic principles of diagnostic microbiology for students preparing to enter an allied health field. Students are led through a series of exercises that allow them to learn basic microbiology techniques and to practice safety in the laboratory and hospital environment. It remains oriented primarily toward meeting the interests and needs of those who will be directly involved in patient care and who wish to learn how microbiological principles should be applied in the practice of their professions.
The authors have emphasised the purposes and function of the clinical microbiology laboratory in the diagnosis of infectious diseases. The exercises illustrate as simply as possible the nature of laboratory procedures used for isolation and identification of infectious agents as well as the principles of asepsis, disinfection and sterilisation. Attention is given to the role of the health professional in regard to appropriate collection of clinical specimens and the applications of aseptic and disinfectant techniques as they relate to patient care.
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PART 1 Basic Techniques of Microbiology
1. The Microscope
2. Handling and Examining Cultures
3. Hanging Drop and Wet-Mount Preparations
4. Simple Stains
5. Gram Stain
6. Acid-Fast Stain
7. Special Stains
8. Culture Media
9. Streaking Technique to Obtain Pure Cultures
10. Pour-Plate and Subculture Techniques
11. Culturing Microorganisms from the Environment
PART 2 Destruction of Microorganisms
12. Moist and Dry Heat
13. The Autoclave
14. Disinfectants
15. Antimicrobial Agent Susceptibility Testing and Resistance
PART 3 Diagnostic Microbiology in Action
16. Primary Media for Isolation of Microorganisms
17. Some Metabolic Activities of Bacteria
18. Activities of Bacterial Enzymes
19. Principles of Antigen Detection and Nucleic Acid Assays for Detection and Identification of Microorganisms.
20. Staphylococci
21. Streptococci, Pneumococci, and Enterococci
22. Haemophilus, Corynebacteria, and Bordetella
23. Clinical Specimens from the Respiratory Tract
24. The Enterobacteriaceae (Enteric Bacilli)
25. Clinical Specimens from the Intestinal Tract
26. Urine Culture Techniques
27. Neisseria and Spirochetes
PART 4 Microbial Pathogens Requiring Special Laboratory Techniques; Serological Identification of Patients’ Antibodies
28. Anaerobic Bacteria
29. Mycobacteria
30. Mycoplasmas, Rickettsiae, Chlamydiae, Viruses, and Prions
31. Fungi: Yeast and Molds
32. Protozoa and Animal Parasites
33. Serodiagnosis of Infectious Disease
PART 5 Applied (Sanitary) Microbiology
34. Bacteriological Analysis of Water
35. Bacteriological Analysis of Milk
Book by Granato Paul A PhD Morton Verna Mizer Helen Ecke
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