A Practical Guide to the Study of Calcium in Living Cells: Volume 40 - Brossura

Brossura

ISBN 10: 0125228104 ISBN 13: 9780125228107

Casa editrice: Academic Press, 1994

Riassunto

A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don't's and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation.

• Describes techniques for detection of [Ca²⁺]I: Ca²⁺ - sensitive microelectrodes
• Fluorescent dyes
• Luminescent proteins
• Includes techniques for perturbing intracellular Ca²⁺
• Covers detailed methodology plus problems and pitfalls of each technique
• Contains a practical guide to preparing Ca²⁺ buffers with an easy-to-use computer program
• Color plates illustrate techniques such as
• Confocal ratio-imaging
• Use of aequorin

Su questo libro

Dalla quarta di copertina

This volume in the acclaimed series Methods in Cell Biology is designed for students and researchers in biology and biomedicine. A Practical Guide to the Study of Calcium in Living Cells describes highly sensitive techniques for measuring the calcium vital to many important cellular functions. This manual enables investigators to evaluate confocal images, use the latest dyes, and design Ca²⁺ buffers appropriate to their research needs.
Highlights
* Describes techniques to
* Detect Ca²⁺ with sensitive microelectrodes, fluorescent dyes, and luminescent proteins
* Perturb intracellular Ca²⁺
* Prepare Ca²⁺ buffers with an easy-to-use computer program

* Contains color plates that illustrate confocal ratio-imaging and the use of aequorin
* Covers methods in detail and describes the problems and pitfalls of each technique

Dettagli dell'edizione

• Editore: Academic Press
• Data di pubblicazione: 1994
• Lingua: Inglese
• ISBN 10: 0125228104
• ISBN 13: 9780125228107
• Rilegatura: Rilegatura a spirale
• Numero di pagine: 368
• Redattore: Nuccitelli Richard
• Contatto del produttore: non disponibile
• Persona responsabile: non disponibile