Brings together a selection of key articles from Volumes 216, 217, and 218 of Methods in Enzymology , providing the most essential and widely used procedures for graduate students and researchers. Methods are included for DNA isolation and cloning, synthesizing complementary DNA (cDNA), cleaving and manipulating DNA, selecting useful reporter genes, constructing vectors for cloning genes, constructing expression vectors, site-directed mutagenesis and gene disruption, identifying and mapping genes, transforming animal and plant cells, sequencing DNA, amplifying and manipulating DNA by PCR, and detecting DNA-protein interaction. Plastic comb-binding. Annotation c. by Book News, Inc., Portland, Or.
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