This volume focuses on procedures used in the isolation of polypeptide hormones and their identification and structural analysis; the development of sensitive bioassay and immunoassay techniques; methods for tissue localization of peptide hormones and their encoding MRNAs; and techniques for characterization of peptide hormone receptors.
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'This volume is very clearly laid out with a good index, an excellent supply of up-to-date references and clear illustrations. It is an excellent introduction to the area and a valuable laboratory book for its procols.' Cell Biology International Reports, Vol. 15, No.8, 1991
'... this text will draw to the attention of those working in one corner of the peptide hormone field some of the methodologies used by those working in different corners of the same field and should benefit all concerned.' FEBS Letters
'There is a companion volume on peptide hormone action. Both volumes provide excellent practical details and will be very useful to bench workers.' International Journal of Biochemistry
C. McLean: Peptide hormone purification; P. Jackson: Electrophoretic and immunoblotting methods; R. Edwards: Radioimmunoassay; K. Siddle: Immunometric assays; W.R. Robertson: The In vitro bioassay of peptide hormones; P. C. Emson, D.J. Nunez, & A.P. Davenport: In situ hybridization for the localization and quantification of peptide mRNAs; A. Clark & D. Pow: Localization of peptide hormones by light and electron microscopy; W.J. Malaisse, V. LeClerq-Meyer, & F. Malaisse-Lagae: Methods for the measurement of insulin secretion; K.W. Brocklehurst & H. Pollard: Cell biology of secretion; P.E.R. Tatham & B. Gomperts: Cell permeabilization; D.S.W. Boam, K. Docherty, K.I.J. Shennan, & A. Clark: The molecular biology of polypeptide hormone expression and biosynthesis; J.C. Hutton, E.M. Bailyes, C.J. Rhodes, & P.C. Guest: Regulation of the biosynthesis and processing of polypeptide hormones
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