Receptor Biochemistry: A Practical Approach (Practical Approach Series): 65 - Brossura

Brossura

ISBN 10: 0199630933 ISBN 13: 9780199630936

Casa editrice: Oxford University Press, USA, 1990

Riassunto

The acquisition of three-dimensional structural information about receptors, particularly through the application of crystallographic and other physical techniques requires the purification and handling of significant amounts of receptor proteins. This book focuses particularly on strategies which have proven successful in the purification of a number of receptors of pharmacological importance. Included are examples of C-protein-coupled receptors, oligomeric receptors which incorporate an integral ion channel, and receptor tyrosine kinases. Specific aspects include the choice of detergents for solubilization, the synthesis and utilization of specific affinity gels, and peptide mapping, among other key topics. There is also an introduction to structure prediction from receptor sequences, and the high-level expression of cloned receptor genes. The book draws on the experience and insight of a number of scientists who have been at the forefront of progress in this difficult and experimentally demanding field.

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Recensione

'obviously useful ... Cross referencing is good and the book has been well organized so that generalisations appear convincing and principles emerge. In this sense, it is a very well-structured receipe book.' Dr. K.E. Mogensen, Institut de Recharches Scientifiques sur le Cancer, Villejuif, France, Methods in Molecular and Cellular Biology

'Cross referencing is good and the book has been well organized so that generalisations appear convincing and principles emerge ... it is a very well-structured recipe book.'Methods in Molecular and Cellular Biology

'This volume will be a valuable resource of proven protocols, expert guidance, and insightful comment.'Anticancer Research 12: (1992)

`This volume will be a valuable resource of proven protocols, expert guidance, and insightful coment, drawn from the experience of researchers at the forefront of this difficult and experimentally demanding field.'Anticancer Research

Product Description

This book is one of a trio of volumes covering practical aspects of neurotransmitter and hormone-receptor-ligand interactions, receptor biochemistry and receptor-effector coupling. The acquisition of 3-dimensional structural information about receptors, particularly through the application of crystallographic and other physical techniques, will require the purification and handling of significant amounts of receptor proteins. This book focuses particularly on strategies which have proved successful in the purification of a number of receptors of pharmacological importance. Included are examples of G-protein coupled receptors, oligomeric receptors which incorporate an integral ion channel, and receptor tyrosine kinases. Specific aspects covered include the choice of detergents for solubilization, the synthesis and utilization of specific affinity gels, the characterization of purified receptor proteins and techniques for specific polypeptide cleavage, peptide mapping and the isolation of sequenceable peptides. There is also an introduction to structural prediction from receptor sequences, and the high-level expression of cloned receptor genes. The book draws on the practical experience and insight of a number of scientists who have been at the forefront of progress in this difficult and demanding field.

Dettagli dell'edizione

• Editore: Oxford University Press, USA
• Data di pubblicazione: 1990
• Lingua: Inglese
• ISBN 10: 0199630933
• ISBN 13: 9780199630936
• Rilegatura: Copertina flessibile
• Numero di pagine: 346
• Redattore: Hulme E. C.
• Contatto del produttore: non disponibile
• Persona responsabile: Ufficio Anagrafiche Messaggerie; ufficio.anagrafiche@meli.it; ; Via Verdi, 8; Assago Milano; 20090; Italia