Directed Mutagenesis: A Practical Approach: 73 - Brossura

Brossura

ISBN 10: 0199631409 ISBN 13: 9780199631407

Casa editrice: IRL Press, 1991

Riassunto

Directed mutagenesis has emerged as an essential tool in both genetic and protein engineering by facilitating both molecular analysis and the modification of the structural and functional properties of cloned DNA sequences. This book draws together a wide range of methods for the efficient directed mutagenesis of cloned DNA sequences.

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Recensione

'The text is presented in such a way as to be suitable for both beginner and expert, especially those interested in exploring alternative procedures.' ASLIB Booklist, Vol.56, No.10, October 1991

'This book draws together a wide range of methods for the efficient directed mutagenesis of cloned DNA sequences. It provides up-to-date protocols and practical advice that will prove valuable to the beginner who wishes to be guided through the available approaches, and to the experienced researcher interested in exploring alternative procedures.'Biochimica Clinica, 1992, Vol. 16, No. 6

'The latest in the Practical Approach series ... is all that you would expect from its long line of predecessors. An illustrious list of contributors includes many leaders in their respective fields as well as the pioneers of the techniques they describe. This alone lends authority to and instils confidence in each method.'Daniel C. Emery, University of Bristol, BioEssays, Vol. 15, No. 4 - April 1993

Contenuti

Paul Carter: Mutagenesis Facilitated by the Removal or Introduction of Unique Restriction Sites; P. D. Yuckenberg, F. Witney, J. Geisselsoder, & J. McClary: Efficient Site-directed In Vitro Mutagenesis using Uracil-containing DNA and Phagemid Vectors; J. Sayers & F. Eckstein: Phosphorothioate-based Site-directed Mutagenesis for Single-stranded Vectors; S Inouye & M. Inouye: Site-specific Mutagenesis using Double-stranded Plasmid DNA; D.M. Olsen & F. Eckstein: Phosphorothioate-based Double-stranded Plasmid Mutagenesis; B. Luckow & G. Shutz: Linker Scanning Mutagenesis; C. Walton, R.K. Booth, & P.G. Stockley: Random Chemical Mutagenesis and the Non-selective Isolation of Mutated DNA Sequences In Vitro; J.K.C. Knowles & P. Lehtovaara: An Enzymatic Method for the Complete Mutagenesis of Genes; S.C. Blacklow & J.R. Knowles: Spiked Oligonucleotide Mutagenesis; J.H. Richards: Cassette Mutagenesis; R.M. Horton & L.R. Pease: Recombination and Mutagenesis of DNA Sequences using PCR

Dettagli dell'edizione

• Editore: IRL Press
• Data di pubblicazione: 1991
• Lingua: Inglese
• ISBN 10: 0199631409
• ISBN 13: 9780199631407
• Rilegatura: Copertina flessibile
• Numero di pagine: 278
• Redattore: McPherson M. J.
• Contatto del produttore: non disponibile
• Persona responsabile: Ufficio Anagrafiche Messaggerie; ufficio.anagrafiche@meli.it; ; Via Verdi, 8; Assago Milano; 20090; Italia