Protein Targeting: A Practical Approach: 101 - Rilegato

Rilegato

ISBN 10: 0199632103 ISBN 13: 9780199632107

Casa editrice: IRL Press, 1992

Riassunto

It has been known for many years that the subcellular distribution of proteins throughout eukaryotic cells is highly ordered. This means that proteins have to be transported to their correct destinations and left there. Understanding the complex mechanisms by which this occurs has provided a major challenge for modern biology.

Recent progress in the subject has relied heavily on finding ways to measure protein transport and targeting. Protein Targeting brings together contributions from active researchers in the field, who provide detailed step-by-step protocols for studying the processes involved, using both intact cells and broken cell preparations. Cell biological, biochemical, and molecular biological methods are all addressed in detail, and the chapters cover the well-established techniques, as well as a number of exciting new methodologies which have potentially very wide applications. This book will enable scientists from varied backgrounds to get to grips with unravelling the mechanisms which control cellular protein targeting.

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Recensione

'the topics covered by the chapters in this book are a well-balanced choice ... The text is clear and concise ... the contributions from active researchers in the field make this book a valuable addition to both the laboratory and library bookshelf.'Trends in Biochemical Sciences, Volume 18, No. 5, May 1993

'This new addition to the Practical Approach series is filled with easy to follow, yet comprehensive, practical protocols that cover a variety of modern molecular cell-biological techniques with which to study this exciting and complex area of cell function. This book will no doubt prove of great value to those working in any area of protein targeting.'N.M. Hooper, Biochemical Education 21 (3) 1993

Contenuti

C.M. Nolan: Receptor-mediated endocytosis and lysosomal transport; J.A. Ellis, M.R. Jackman, J.H. Perez, B.M. Mullock, J.P. Luzio: Membrane traffic pathways in polarized epithelial cells; L.P. Cramer & D.F. Cutler: Sorting between exocytic pathways in PC12 cells; J. Armstrong, E. Fawell, & A. Pidoux: Intracellular trafficking in fission yeast; D. Vaux: Network antibodies as tools for studying intracellular protein traffic; U. Wienhues, H. Koll, K. Becker, B. Guiard, & F-U. Hartl: Protein targeting to mitochondria; C. Dingwall: Techniques in nuclear protein transport; J. Gruenberg, & J.P. Gorvel: In vitro reconstruction of endocytic vesicle fusion; R. Schwaninger: In vitro reconstitution of vesicular transport from the endoplasmic reticulum to the cis Golgi in semi-intact cells; W.J. Masterson, & A.I. Magee: Lipid modifications involved in protein targeting.

Dettagli dell'edizione

• Editore: IRL Press
• Data di pubblicazione: 1992
• Lingua: Inglese
• ISBN 10: 0199632103
• ISBN 13: 9780199632107
• Rilegatura: Copertina rigida
• Numero di pagine: 288
• Redattore: Magee Anthony I., Wileman Thomas
• Contatto del produttore: non disponibile
• Persona responsabile: Ufficio Anagrafiche Messaggerie; ufficio.anagrafiche@meli.it; ; Via Verdi, 8; Assago Milano; 20090; Italia