Extracellular Matrix: A Practical Approach: 151 - Brossura

Brossura

ISBN 10: 0199632200 ISBN 13: 9780199632206

Casa editrice: IRL Press, 1993

Riassunto

The extracellular matrix surrounds cells and tissues. It provides structure to organisms and is important in determining cell growth, state of differentiation, and how the cell responds during injury and development. Changes in the extracellular matrix are also important components in both inherited and acquired diseases. Thus studies of the extracellular matrix span much of modern biological research. Because the extracellular matrix resides outside the cell, the properties of the component molecules require special approaches for their study. This volume brings together under a single cover the majority of the basic techniques employed in extracellular matrix research.

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Recensione

valuable ... It is a multi-authored volume in the superb Practical Approach series and comes packed with protocols on every aspects of ECM research. This is the ECM book that has been needed for a long time, and here are essential protocols for any groups with even the slightest interest in the ECM. I recommend it highly. (Charles Streuli, University of Manchester, Trends in Cell Biology, (Vol.6), November 1996)

This book sets out to provide the research worker with a comprehensive survey and detailed protocols of the biochemical techniques used for studying the macromolecules of the extracellular matrix. The senior authors of all the chapters are well established in the field of their contribution. This is a strong recommendation for a book which should become a standard handbook for anyone working in this field, particularly those just entering or wishing to develop new techniques. (Doreen E. Ashhurst, St George's Hospital Medical School, Cell Biology International, Vol. 20, No. 9, 1996)

Contenuti

• 1: Overview of the different collagen types and approaches for the isolation and characterization of types I, III, V and VI molecules from tissues and cultured cells
• 2: Approaches for isolating and characterizing the collagens in cartilage
• 3: Techniques for evaluating the matrix components found in basement membranes
• 4: Approaches for investigating the specialized matrix components produced by endothelial cells
• 5: Techniques for the isolation and characterization of the enzymes involved in the post-translational processing of collagen
• 6: Techniques for the study of fibronectin and tenascin
• 7: Approaches for the study of glycosaminoglycans
• 8: Approaches for the evaluation of proteoglycans
• 9: Techniques for the use of the extracellular matrix and its components in cell culture
• 10: Approaches for investigating elastin
• 11: Techniques for evaluating the catabolism of extracellular matrix components
• 12: Immunological approaches for the identification of extracellular matrix components in tissues and cultured cells
• 13: Molecular biological approaches for studying extracellular matrix production

Dettagli dell'edizione

• Editore: IRL Press
• Data di pubblicazione: 1993
• Lingua: Inglese
• ISBN 10: 0199632200
• ISBN 13: 9780199632206
• Rilegatura: Copertina flessibile
• Numero di pagine: 432
• Redattore: Haralson M. A., Hassell John R.
• Contatto del produttore: non disponibile
• Persona responsabile: non disponibile