Protein Phosphorylation: A Practical Approach (Practical Approach Series): 123 - Brossura

Brossura

ISBN 10: 0199633053 ISBN 13: 9780199633050

Casa editrice: Oxford University Press, USA, 1993

Riassunto

Reversible phosphorylation is one of the major mechanisms by which protein activity is controlled in all eukaryotic cells. Networks of protein kinases and phosphatases, which catalyze phosphorylation and dephosphorylation respectively, are responsible for switching between different pathways of cell division, development, or differentiation in response to hormones or growth factors. This book provides a comprehensive description of current methods for studying protein phosphorylation and the protein kinases and phosphatases which catalyze it. It includes detailed protocols for studying phosphorylation in intact cells, approaches using enzyme inhibitors, purification of serine/threonine-specific protein kinases/phosphatases, analysis of kinase/phosphatase specificity, and cloning relevant cDNAs. Protein Phosphorylation" A Practical Approach will be of great value to all researchers in the fields of intracellular and intercellular signalling, including biochemists, molecular biologists and pharmacologists.

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Recensione

'In one word: a precious book which has to be in all hands.'Cellular and Molecular Biology, Vol. 40/No. 4 June 1994

Contenuti

• 1: J.C. Garrison: Study of protein phosphorylation in intact cells
• 2: P. van der Geer, K. Luo, B.M. Sefton, and T. Hunter: Phosphopeptide mapping and phosphoamino acid analysis on cellulose thin layer plates
• 3: D.G. Hardie, D.G. Campbell, F.B. Caudwell, and T.A.J. Haystead: Analysis of sites phosphorylated in vivo and in vitro
• 4: D.G. Hardie: Use of protein (serine/threonine) kinase activators and inhibitors to study protein phosphorylation in intact cells
• 5: D.G. Hardie: Use of protein phosphatase inhibitors in intact cells
• 6: Y. Wang and P.J. Roach: Purification and assay of mammalian protein (serine/theonine) kinases
• 7: A.A. Stewart: Purification of protein (tyrosine) kinases
• 8: S. Hanks and M.A. Lawton: Homology-based approaches for identifying cDNAs that encode eukaryotic protein (serine-threonine) and protein (tyrosine) kinases
• 9: C. MacKintosh: Assay and purification of protein (serine/threonine) phosphatases
• 10: N.K. Tonks: Characterization of protein (tyrosine) phosphatases
• 11: M.X. Chen and P.T.W. Cohen: Identification of novel protein (serine/threonine) phosphatase genes by saccharomyces cerevisiae, drosophila melanogaster, and man by the polymerase chain reaction
• 12: R.B. Pearson, K.I. Mitchelhill, and B.E. Kemp: Studies of protein kinase/phosphatase specificity using synthetic peptides

Dettagli dell'edizione

• Editore: Oxford University Press, USA
• Data di pubblicazione: 1993
• Lingua: Inglese
• ISBN 10: 0199633053
• ISBN 13: 9780199633050
• Rilegatura: Copertina flessibile
• Numero di pagine: 322
• Redattore: Hardie D. Grahame
• Contatto del produttore: non disponibile
• Persona responsabile: Ufficio Anagrafiche Messaggerie; ufficio.anagrafiche@meli.it; ; Via Verdi, 8; Assago Milano; 20090; Italia