RNA Processing: A Practical Approach: Volume I: 001 - Brossura

Brossura

ISBN 10: 0199633436 ISBN 13: 9780199633432

Casa editrice: IRL Press, 1998

Riassunto

Post-transcriptional events are increasingly recognized as important sites of regulation of gene expression in eukaryotic cells and so RNA processing has rapidly become an area of intense research activity. These two volumes give detailed practical guidance on all the major aspects of this subject. Step-by-step practical protocols from leading laboratories are presented for studies of the termination and end-processing, capping, methylation, splicing, and editing of mRNA as well as studies of mRNA stability and processing of rRNA and tRNA. The reader is led through all the key steps required for a successful experimental investigation. This includes full descriptions of the synthesis and purification of RNA substrates for in vitro work, the characterization of specific RNAs, and the isolation and analysis of ribonucleoprotein complexes.

RNA processing - A Practical Approach, Volumes I and II, are invaluable laboratory companions for researchers working on mRNA, rRNA, or tRNA gene expression. Their lucid explanatory style and comprehensive coverage of all the key techniques for analysing RNA processing will be welcomed by both experienced researchers and

Su questo libro

L'autore

Stephen J. Higgins and B. David Hames are both at the University of Leeds.

Contenuti

• 1: Synthesis and purification of RNA substrates
• 2: Characterization of RNA
• 3: Splicing of mRNA precursors in mammalian cells
• 4: Isolation and characterization of ribonucleoprotein complexes
• 5: Analysis of ribonucleoprotein interactions
• 6: Analysis of pre-mRNA splicing in yeast
• Appendix

Dettagli dell?edizione

• Editore: IRL Press
• Data di pubblicazione: 1998
• ISBN 10: 0199633436
• ISBN 13: 9780199633432
• Rilegatura: Copertina flessibile
• Lingua: Inglese
• Numero di pagine: 232
• Redattore: Higgins Stephen J., Hames B. David
• Contatto del produttore: non disponibile