Sinossi
Post-transcriptional events are increasingly recognized as important sites of regulation of gene expression in eukaryotic cells and so RNA processing has rapidly become an area of intense research activity. These two volumes give detailed practical guidance on all the major aspects of this subject. Step-by-step practical protocols from leading laboratories are presented for studies of the termination and end-processing, capping, methylation, splicing, and editing of mRNA as well as studies of mRNA stability and processing of rRNA and tRNA. The reader is led through all the key steps required for a successful experimental investigation. This includes full descriptions of the synthesis and purification of RNA substrates for in vitro work, the characterization of specific RNAs, and the isolation and analysis of ribonucleoprotein complexes.
RNA processing - A Practical Approach, Volumes I and II, are invaluable laboratory companions for researchers working on mRNA, rRNA, or tRNA gene expression. Their lucid explanatory style and comprehensive coverage of all the key techniques for analysing RNA processing will be welcomed by both experienced researchers and
Le informazioni nella sezione "Riassunto" possono far riferimento a edizioni diverse di questo titolo.
L'autore
Stephen J. Higgins and B. David Hames are both at the University of Leeds.
Contenuti
- 1: Synthesis and purification of RNA substrates
- 2: Characterization of RNA
- 3: Splicing of mRNA precursors in mammalian cells
- 4: Isolation and characterization of ribonucleoprotein complexes
- 5: Analysis of ribonucleoprotein interactions
- 6: Analysis of pre-mRNA splicing in yeast
- Appendix
Le informazioni nella sezione "Su questo libro" possono far riferimento a edizioni diverse di questo titolo.
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RNA Processing: A Practical Approach Volume I: Vol 1 (Practical Approach Series)
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Paperback. Condizione: Very Good. RNA Processing: A Practical Approach Volume I: Vol 1 (Practical Approach Series) This book is in very good condition and will be shipped within 24 hours of ordering. The cover may have some limited signs of wear but the pages are clean, intact and the spine remains undamaged. This book has clearly been well maintained and looked after thus far. Money back guarantee if you are not satisfied. See all our books here, order more than 1 book and get discounted shipping. . Codice articolo 7719-9780199633432
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RNA Processing: A Practical Approach Volume I: Vol 1 (Practical Approach Series)
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RNA Processing: A Practical Approach volume I.
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Hardcover. Condizione: Fine. This two-volume set provides detailed practical guidance on all major aspects of RNA processing. Each procedure is clearly explained so that the reader can follow all of the key stages of a successful experimental investigation. Codice articolo cbc30963-2318-4e09-b676-9455b2e63231
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Rna Processing: A Practical Approach: Vol 001
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