Tumour Immunobiology: A Practical Approach: 121 - Brossura

Brossura

ISBN 10: 019963369X ISBN 13: 9780199633692

Casa editrice: Oxford University Press, USA, 1993

Riassunto

The idea that the immune system may play a role in protecting against malignant disease is not new. Since the earliest days of immunology research, scientists have sought to understand the mechanisms by which the host immune system might recognize cancer cells, and to manipulate these responses for therapeutic gain. It has become clear that the key to immune recognition of tumors lies in the relationship between cells in the microenvironment of the tumor itself. This volume provides a comprehensive collection of techniques for establishing tumor cell lines, testing their immunogenicity, and characterizing the major effector cells, leading to protocols for manipulating antibody and T-cell receptor structure, preparing cytokine-secreting tumor cells and testing the efficiency of these new agents in preventing tumor growth. Researchers in immunology as a whole in addition to those interested in cancer immunology will find this volume to be an invaluable practical resource.

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Recensione

'The editors of this book should be congratulated for ensuring that their contributors maintained a common style of method presentation, from the simplest of procedures such as the removal of a tumour from a tumour-bearing animal, to the more complex, such as modifying chimeric T-cells using chimeric T-cell receptor (TCR)-Ig genes. The instructions for each method are concise, precise and include a list of the equipment required for each procedure. In addition, methods for evaluating the success of a procedure are detailed carefully, so thst the practitioner can evaluate if a technique has been accomplished successfully ... the methods and protocols described within this volume will be extremely useful for the basic tumour immunobiologist, regardless of theirlevel of experience.'Lionel A. Manson, University of Pennsylvania, Immunology Today, Vol. 15, No. 2, 1994

This book provides a comprehensive discussion of the many techniques and approaches essential for the study of tumor immunobiology. Each chapter provides excellent background information to clarify the importance of the technique, approach, or antitumor mechanism which is being discussed. I would strongly recommend this text as a reference for individuals working in tumor immunology as well as for the scientist interested in understanding practical techniques needed to evaluate the immune response to cancer. This book clearly encourages and facilitates additional research in tumor immunology by providing essential tools for investigators interested in this research. (M. Albertini, Oncology, Vol. 52, No. 3, 1995)

Contenuti

• 1: A.P. Wilson: Preparation of tumour cell-lines
• 2: R.R. Miller, B.E. Miller, and G.H. Heppner: Assessment of the immunogenicity of tumour models
• 3: J. Lawry: Flow cytometry of lymphocytes and tumour cells
• 4: M.R. Price: (Glyco-) protein antigens and peptide epitopes of tumours
• 5: T. Timonen, A. Maenpaa, and P. Kovanen (with additional material by V.P. Thorgersson and A.R. Mackay): Isolation and characterization of natural killer cells
• 6: L.J. Partridge and I. Dransfield: Isolation and characterization of mononuclear phagocytes
• 7: R.A. Robins: T-cells: proliferative and cytotoxic responses
• 8: G. Pawelec: Cloning and propagation of human T lymphocytes
• 9: S.L. Hand, B.L. Hall, and O.J. Finn: Association of TCR expression with MHC recognition
• 10: F.S. di Giovine, S. Stones, D. Wojtacha, and G.W. Duff: Cytokines: identification and measurement of gene activation
• n 11F.P. Winstanley: Cytokine bioassay
• 12: M. Kurpisz and G. Wilson: Strategies for the production of human monoclonal antibodies
• 13: M.V. Pimm and S.J. Gibbon: Radiolabelling antibodies for imaging and targeting
• 14: M.J. Glennie, A.L. Tutt, and J. Greenman: Preparation of multispecific F(ab) and F(ab) antibody derivatives
• 15: M.S. Hayden, H.P. Fell, and N.F. Landolfi: Construction and expression of Ig fusion proteins
• 16: M.E. Verhoeyen: Reshaping human monoclonal antibodies for in vivo diagnosis and therapy
• 17: M. Whitlow and D. Filpula: Single-chain Fvs
• 18: P.M. Patel, C.L. Flemming, S.A. Eccles, and M.K.L. Collins: Engineering cytokine-secreting tumour cells
• 19 Ex vivo activation of effector cells: K.E. Platts:
• 20: C. Darnbrough: Genetic immortalization of human lymphocytes using retroviral vectors
• 21: Z. Eshhar, G. Gross, and J. Treisman: Modifying the specificity of T cells using chimeric Ig/TCR genes
• 22: R.B. Wiltrout: The use of pre-clinical rodent models for cancer immunotherapy
• n 23I.E. Grcia de Palazzo, M. Holmes, K. Alpaugh, and L.M. Weiner: Use of the tumour spheroid model in immunotherapy

Dettagli dell'edizione

• Editore: Oxford University Press, USA
• Data di pubblicazione: 1993
• Lingua: Inglese
• ISBN 10: 019963369X
• ISBN 13: 9780199633692
• Rilegatura: Copertina flessibile
• Numero di pagine: 448
• Redattore: Gallagher G., Rees R. C., Reynolds C. W.
• Contatto del produttore: non disponibile
• Persona responsabile: Messaggerie Libri ; ufficio.anagrafiche@meli.it; ; Via Verdi, 8; Assago Milano; Italia