Glycobiology: A Practical Approach: 125 - Brossura

Brossura

ISBN 10: 0199633711 ISBN 13: 9780199633715

Casa editrice: IRL Press, 1994

Riassunto

The interest in carbohydrates of glycoproteins has greatly intensified in the past few years. The recent development of recombinant glycoproteins for therapeutic purposes necessitates the understanding of the structure-function relationship in glycoproteins has significantly expanded. For instance, it is now known that carbohydrate-protein interaction is one of the most critical events in leukocytendothelial cell interaction. Such developments in biology and medicine require an updated methodology that aims at the basic understanding of the structure and biosynthesis of glycoproteins. In this book, the chapters are assembled to provide basic background and methods in order to allow one to analyse the structures of glycoproteins. The subjects covered include isolation, fractionation and structural characterization of typical glycoproteins, such as those containing N-glycans. The book also covers the phosphatidylinositol anchor, a new type of glycosylation to attach proteins to membranes.

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Contenuti

• 1: Isolation and characterization of glycoproteins
• 2: Compositional analysis of glycoproteins
• 3: Isolation and fractionation of N-linked oligosaccharides from glycoproteins
• 3a: Release of N-linked oligosaccharides and fractionation by gel filtration
• 3b: Fractionation of oligosaccharides by serial affinity chromatography with use of immobilized lectin columns
• 3c: Fractionation of glycopeptides and oligosaccharides from glycoproteins by HPLC
• 4: Structural characterization of oligosaccharides from glycoproteins
• 4a: Glycosidase treatment and other methods, including methylation analysis
• 4b: FAM-MS and ES-MS of glycoproteins
• 4c: Nuclear magnetic resonance spectroscopy of glycoprotein-derived carbohydrate chains
• 5: Structural characterization of N-glycans obtained from metabolically-radiolabelled glycoproteins
• 6: Structural characterization of mucin-type O-linked oligosaccharides
• 7: Characterization of nuclear and cytoplasmic glycoproteins
• 8: GPI membrane anchors
• Appendix
• Index

Dettagli dell?edizione

• Editore: IRL Press
• Data di pubblicazione: 1994
• Lingua: Inglese
• ISBN 10: 0199633711
• ISBN 13: 9780199633715
• Rilegatura: Copertina flessibile
• Numero di pagine: 432
• Redattore: Fukuda Minoru, Kobata Akira
• Contatto del produttore: non disponibile
• Persona responsabile: Ufficio Anagrafiche Messaggerie; ufficio.anagrafiche@meli.it; ; Via Verdi, 8.; Assago Milano; 20090; Italia