The Cell Cycle: A Practical Approach (Practical Approach Series): 137 - Brossura

Brossura

ISBN 10: 0199633940 ISBN 13: 9780199633944

Casa editrice: Oxford University Press, U.S.A., 1994

Riassunto

The use of recombinant D.N.A. and related technologies has revealed the extraordinary degree to which cell cycle control mechanisms have been conserved through eukaryotic evolution. The availability of genes and gene products derived from different organisms has greatly extended what is experimentally possible. As a result, many researchers are now faced with the need to handle a far wider range of organisms and must therefore be acquainted with the ever-increasing arsenal of experimental techniques. This book is a compilation of key techniques currently used in the most popular experimental systems. The book describes how specific organisms or cell types are handled and focuses on particular techniques that are applicable to any cell type. A wide range of biochemical, genetic, and cellular manipulations are described. Students and researchers in cell and molecular biology will welcome this accessible, useful new guide.

Su questo libro

Recensione:

'the idea of putting these techniques together in one volume is an excellent one ... The overall impression, however, is that this manual is a useful theoretical and practical introduction for anyone itnerested in current eukaryotic cell cycle research. Battered copies will soon be found on the shelves of many laboratories working on cell cycle issues.'Rolf Bernander, The Norwegian Radium Hospital, Trends in Cell Biology, Vol. 4, October 1994

Contenuti:

• 1: The synchronization of mammalian cells
• 2: Preparation of synchronous cultures of the yeasts Saccharomyces cerevisiae and Schizosaccaromyces pombe
• 3: Mammalian cell cycle analysis
• 4: Analysis of the cell cycle in Saccharomyces cerevisiae
• 5: Methods for the analysis of the fission yeast cell cycle
• 6: Cell cycle analysis using the filamentous fungus Aspegillus nidulans
• 7: Techniques for studying mitosis in Drosophila
• 8: The use of cell-free extracts of Xenopus eggs for studying DNA replication in vitro
• 9: In vitro SV40 DNA replication
• 10: Analysis of DNA replication origins and directions by two-dimensional gel electrophoresis
• 11: The isolation of functional mitotic organelles from tissue culture cells
• 12: Frog eggs as a system to study mitosis
• 13: Maturation-promoting factor and cyclin-dependent kinases

Su questa edizione

• Editore: Oxford University Press, U.S.A.
• Data di pubblicazione: 1994
• ISBN 10: 0199633940
• ISBN 13: 9780199633944
• Rilegatura: Copertina flessibile
• Numero di pagine: 352
• Redattore: Fantes Peter