PCR (polymerase chain reaction) is now one of the most widely used of basic molecular biology techniques and is an indispensable research tool for the molecular biologist. The basic PCR technique provides the cornerstone for in vitro DNA amplification - allowing the researcher to produce large quantities of DNA from minute amounts of starting material.PCR: A Practical Approach Volume 2 is not a revised version of PCR: A Pracical Approach, but sets out to address some of the exciting new applications fo PCR including cDNA cloning, mRNA quantitation and expression of proteins from PCR products, genomic DNA mapping and fingerprinting, and mutational analysis.
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This book gives a good insight into the more advanced PCR technology avaiable and will certainly be a useful addition to laboratories wishing to expand their PCR repertoire. (Dr Amanda Evans, Pharma Research, CIBA AG, Basel, Cell Biology International, 1996, Vol. 20, No. 6)
B. D. Hames, Department of Biochemistry, both at the University of Leeds. G.R. Taylor, Regional DNA Laboratory, Leeds.
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