This work is designed to provide research workers with detailed protocols for studying bacterial plasmids, and for using both plasmids and phagemids as vectors. The techniques for studying plasmid replication and maintenance are described in detail, as are fundamental methods for purifying plasmids and for introducing them into cells. Also included are protocols for mutagenesis, and for analyzing plasmid-encoded products. Other chapters focus on techniques for studying plasmids and for using plasmid vectors in particularly important groups of bacteria - animal and plant pathogens harbouring virulence plasmids, streptomyces and lactococci. Plasmid composite vectors and phagemids of various kinds are useful for expression, cDNA cloning and other genetic manipulations. The protocols for using them, as well as important yeast shuttle vectors are described in detail.
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Hardcover. Condizione: Good. No Jacket. Former library book; Pages can have notes/highlighting. Spine may show signs of wear. ~ ThriftBooks: Read More, Spend Less. Codice articolo G0199634459I3N10
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Hardcover. 2nd ed. xviii, 252 S. Ehem. Bibliotheksexemplar mit Signatur und Stempel. GUTER Zustand, ein paar Gebrauchsspuren. Ex-library with stamp and library-signature. GOOD condition, some traces of use. R14515 9780199634453 Sprache: Deutsch Gewicht in Gramm: 550. Codice articolo 2470798
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