DNA Cloning: A Practical Approach Volume 1: Core Techniques (Practical Approach, No 148) - Brossura

 
9780199634767: DNA Cloning: A Practical Approach Volume 1: Core Techniques (Practical Approach, No 148)

Sinossi

DNA Cloning: A Practical Approach will build into an indispensable four volume set offering a complete guide to major techniques and protocols required by modern molecular biology laboratories. This new massively expanded edition is a complete update of Hames and Glover's original major two volume set - focusing on new techniques.

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Contenuti

  • 1.: Techniques for transformation of E.coli
  • 2.: Lambda replacement vectors in the construction of genomic DNA libraries
  • 3.: Procedures for cDNA cloning
  • 4.: Making probes
  • 5.: Using cloned DNAs to analyse gene organization and expression
  • 6.: Choices of single-stranded vectors and their application for in vitro mutagenesis
  • 7.: PCR and its applications
  • 8.: DNA sequencing

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