Platelets: A Practical Approach: 167 - Brossura

Brossura

ISBN 10: 0199635374 ISBN 13: 9780199635375

Casa editrice: IRL Press, 1997

Riassunto

Platelets are small blood cells that play a vital role in blood clotting. They are essential for wound healing, but their unwanted action can lead to ailments such as stroke and heart attack. In seventeen chapters from leading experts in platelet research this book describes state-of-the-art techniques for the experienced researcher, both in academia and industry. Methodology is also included that will enable the newcomer to perform routine experiments. Techniques covered range from basic procedures such as platelet preparation to the latest techniques such as monitoring calcium levels in single cells, protein tyrosine phosphorylation, and PCR.

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Recensione

recent addition to the well known and much respected Practical Approach Series. ... In common with the other volumes in the series, the book provides detailed and comprehensive laboratory protocols for the study of all aspects of platelet function. ... This is not a book for the merely curious, however. it is aimed squarely at those with a determined research interest in platelet function or signal transduction. ... The clear and crisp descriptions are sufficient to allow a comparative novice in the field to establish the methods with relative ease. ... Platelets should prove useful to anyone with an interest in the biochemistry of cellular signal transduction. (British Journal of Biomedical Science 1997)

Contenuti

• Preface
• Acknowledgement
• 1: Platelet preparation and functional responses
• 2: Cellular model systems to study megakaryocyte differentiation
• 3: Techniques in the preparation of semi-permeable platelets and their uses
• 4: Use of fluorescent indicators to measure intracellular Ca2+ and other ions
• 5: Preparation of highly purified human platelet plasma and intracellular membranes using high voltage free flow electrophoresis and methods to study Ca2+ regulation
• 6: The use of flow-cytometry to study platelet activation
• 7: Identification, isolation, and characterization of platelet glycoproteins mediating platelet adhesion
• 8: Monoclonal antibodies to platelet cell surface antigens and their use
• 9: Measurement of protein phosphorylation, kinase activity, and G protein function in intact platelets and membrane preparations
• 10: Tyrosine phosphorylation
• 11: Study of proteins associated with the platelet cytoskeleton
• 12: Methods for measuring agonist-induced phosphlipid metabolism in intact human platelets
• 13: Cyclic nucleotides: measurement and function
• 14: Immunocytochemical and electron microscope studies of platelets
• 15: Clinical aspects of platelet function
• 16: Platelet procoagulent activity and its measurement
• 17: The study of platelet function in other species
• List of suppliers
• Index

Dettagli dell'edizione

• Editore: IRL Press
• Data di pubblicazione: 1997
• Lingua: Inglese
• ISBN 10: 0199635374
• ISBN 13: 9780199635375
• Rilegatura: Copertina flessibile
• Numero di pagine: 396
• Redattore: Watson Steve P., Authi Kalwant S.
• Contatto del produttore: non disponibile
• Persona responsabile: Ufficio Anagrafiche Messaggerie; ufficio.anagrafiche@meli.it; ; Via Verdi, 8.; Assago Milano; 20090; Italia