Affinity Separations: A Practical Approach: 179 - Rilegato

Rilegato

ISBN 10: 0199635501 ISBN 13: 9780199635504

Casa editrice: IRL Press, 1999

Riassunto

Affinity Separations: A Practical Approach is a new collection of affinity-based purification protocols with an international authorship from across the life sciences. The introductory chapter outlines recent advances in ligand-coupling, matrix choice, and operating modes. The second chapter describes methods for obtaining quantitative data from affinity processes. There are specific chapters describing methods for the affinity separation of proteins, nucleic acids, and oligosaccharides. Methods for immunoglobulin purification and immunoaffinity protocols are discussed in a further chapter, and the use of affinity tags in the recovery of engineered proteins in another. Finally, modern techniques for cell separations are outlined. The book presents detailed laboratory protocols together with troubleshooting advice and reviews of recent developments. It is aimed at bench scientists, both those in analytical research laboratories, and those non-specialists looking to apply affinity methods in their work.

Su questo libro

Contenuti

• 1: Recent developments in affinity separation technologies
• 2: Quantitative affinity chromatography
• 3: Affinity separations of proteins
• 4: Affinity separation of nucleic acids
• 5.: Affinity chromatography of oligosaccharides and glycopeptides
• 6: Immunoaffinity and IgG receptor technologies
• 7: Designer affinity purifications of recombinant proteins
• 8: Affinity separations of cells
• Appendix: list of suppliers

Dettagli dell'edizione

• Editore: IRL Press
• Data di pubblicazione: 1999
• Lingua: Inglese
• ISBN 10: 0199635501
• ISBN 13: 9780199635504
• Rilegatura: Copertina rigida
• Numero di pagine: 272
• Redattore: Matejtschuk Paul
• Contatto del produttore: non disponibile
• Persona responsabile: Ufficio Anagrafiche Messaggerie; ufficio.anagrafiche@meli.it; ; Via Verdi, 8.; Assago Milano; 20090; Italia