Arabidopsis: A Practical Approach: 223 - Rilegato

Rilegato

ISBN 10: 019963565X ISBN 13: 9780199635658

Casa editrice: OUP Oxford, 2000

Riassunto

Arabidopsis has long been acknowledged as the 'Botanical Drosophila' with its small genome, low levels of repetitive DNA, small size and fast generation time it is an ideal molecular genetic tool for the analysis of development in higher plants. Arabidopsis: A Practical Approach provides an introduction to most of the key techniques required for the use of Arabidopsis as an experimental system. It gives a basic introduction to the optimal growth conditions and genetic resources available for Arabidopsis, how this material should be handled, maintained and used. Individual chapters describe strategies for the identification, mapping (using multi-marker lines and recombinant inbreds), and characterisation of different mutants by microscopy, molecular cytogenetics and gene expression analysis. Different cloning strategies, using transposons, T-DNA and map position are described in detail. Sequencing of the Arabidopsis genome will be completed in 2000 and bioinformatics are of key importance; the tools that are available and where they can be found on the Web are presented.

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Recensione

"... valuable for scientists who are beginning to analyse mutations in Arabidopsis."Journal of plant Physiology

Contenuti

• Growth, maintenance and use of Arabidopsis genetic resources
• Preservation and handling of stock centre clones
• Genetic mapping using recombinant inbred lines
• Arabidopsis mutant characterisation: microscopy, mapping and gene expression analysis
• Classical and molecular cytogenetics of Arabidopsis
• Tissue culture, transformation and transient gene expression in Arabidopsis
• Transposon and T-DNA mutagenesis
• Map based cloning in Arabidopsis
• YACs, BACs, cosmids and nucleotide sequences
• Web-based bioinformatic tools for Arabidopsis researchers

Dettagli dell?edizione

• Editore: OUP Oxford
• Data di pubblicazione: 2000
• ISBN 10: 019963565X
• ISBN 13: 9780199635658
• Rilegatura: Copertina rigida
• Lingua: Inglese
• Numero di pagine: 296
• Redattore: Wilson Zoe
• Contatto del produttore: non disponibile