Cytokines: A Practical Approach (Practical Approach Series): 155 - Brossura

Brossura

ISBN 10: 0199635668 ISBN 13: 9780199635665

Casa editrice: Oxford University Press, USA, 1995

Riassunto

Cytokines are important mediators of immunity, inflammation, and cell growth and are relevant to many areas of biomedical research. This book, now in its second edition, provides detailed up-to-date practical information for the study of cytokines and their receptors, including guides to many of the most useful current techniques in cell and molecular biology, immunology, and biochemistry. In all it contains 184 protocols, providing a comprehensive resource for researchers in many disciplines of biomedical research. This book will be essential for graduate students, researchers and professionals in immunology, infectious diseases, autoimmunity, oncology and biomedical research.

Su questo libro

Contenuti

• 1: Cloning and expression of cytokine genes
• 2: Southern blotting of cytokine genes
• 3: Northern analysis, ribonuclease protection and in situ analysis of cytokine messenger RNA
• 4: Polymerase chain reaction to quantitate cytokine mRNA
• 5: Comparative polymerase chain reaction
• 6: Receptor binding studies
• 7: Measurement of cytokine induction of PC-specific phospholipase C and sphingomyelinases
• 8: Two dimensional gel elctrophoresis for purification, sequencing and identification of cytokine-induced proteins in normal and malignant cells
• 9: Antiviral activity of cytokines
• 10: Assays for human B cell responses to cytokines
• 11: T cells and cytokines
• 12: The generation and quantitation of cell-mediated toxicity
• 13: Assays for chemotaxis
• 14: Cytokine regulation of endothelial cells
• 15: Assays for haematopoietic growth factors
• 16: Assays for macrophage activation by cytokines
• 17: Measurement of proliferative, cytolytic and cytostatic activity of cytokines
• 18: Production of cytokine transgenic and knockout mice
• 19: Development of antibodies to cytokines
• 20: Visualizing the production of cytokines and their receptors by single human cells
• 21: Quantitative biological assays for individual cytokines
• 22: RIA, IRMA and ELISA assays for cytokines and their soluble receptors

Dettagli dell'edizione

• Editore: Oxford University Press, USA
• Data di pubblicazione: 1995
• Lingua: Inglese
• ISBN 10: 0199635668
• ISBN 13: 9780199635665
• Rilegatura: Copertina flessibile
• Numero edizione: 2
• Numero di pagine: 454
• Redattore: Balkwill Frances R.
• Contatto del produttore: non disponibile
• Persona responsabile: Ufficio Anagrafiche Messaggerie; ufficio.anagrafiche@meli.it; ; Via Verdi, 8; Assago Milano; 20090; Italia