Cell Growth and Apoptosis: A Practical Approach: 159 - Brossura

Brossura

ISBN 10: 0199635684 ISBN 13: 9780199635689

Casa editrice: IRL Press, 1995

Riassunto

This book offers a unique combination of succinctly expressed basic concepts of cell growth and cell death, particularly by apoptosis, and detailed instructions and protocols on how accurately to measure these processes. Experts in each line of these investigations describe a range of techniques which will enable weve an inexperienced reader to determine with precision such cellular events as the cell cycle traverse, chromosomal abnormalities detected at cell division, DNA damage that causes these abberrations, and cell death, or cell senescence. Establishment of the cell lines for studies is illustrated with reference to leukaemic cells, and optimization of cell proliferation by growth factors is also described. Together, these contributions form a useful laboratory manual for studies of growth abnormalities in human cell.

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Recensione

Is there a market for this book? The answer is a questionable yes ... a good buy. (T. Cotter, Br. J. Biomed Sci 1996)

the book is very readable, and, from the point of view of the quality/price ratio, is a good value for money ... This manual will be undoubtedly very useful to many scientists that sooner or later will finish to work in apoptosis, even if their initial aims were to work in cell proliferation. (Javier Naval and Alberto Anel, Universidad de Zaragoza, FEBS Letters 408 (1997))

Contenuti

• 1: Measuring parameters of growth
• 2: Cell cycle analysis by flow cytometry
• 3: Analysis of flow cytometric DNA histograms
• 4: Determination of the cell proliferative fraction in human tumors
• 5: Chromosome analysis in cell culture
• 6: Assessment of DNA damage in mammalian cells by DNA filter elution methodology
• 7: Morphological and biochemical criteria of apoptosis
• 8: Analysis of cell death by flow cytometry
• 9: Assays of cell growth and cytotoxicity
• 10: Cell synchronization as a basis for investigating control of proliferation in mammalian cells
• 11: Growth and activation of human leukaemic cells in vitro and their growth in the SCID mouse model
• 12: Senescence and immortalization of human cells
• 13: Analysis of the effects of purified growth factors on normal human fibroblasts

Dettagli dell'edizione

• Editore: IRL Press
• Data di pubblicazione: 1995
• Lingua: Inglese
• ISBN 10: 0199635684
• ISBN 13: 9780199635689
• Rilegatura: Copertina flessibile
• Numero di pagine: 296
• Redattore: Studzinski George P.
• Contatto del produttore: non disponibile
• Persona responsabile: Ufficio Anagrafiche Messaggerie; ufficio.anagrafiche@meli.it; ; Via Verdi, 8; Assago Milano; 20090; Italia