Articoli correlati a Essential Molecular Biology Vol 2: 255

Essential Molecular Biology Vol 2: 255 - Brossura

 
9780199636440: Essential Molecular Biology Vol 2: 255

Sinossi

The two Essential Molecular Biology books in the Practical Approach Series are designed for the absolute beginner at gene cloning whether they be at the start of their career or an experienced researcher in another field. As with the first editions, the objective of both volumes is to combine solid practical information with sufficient background material to ensure that the novice can understand how a technique works, what it achieves, and how to make modifications to suit personal requirements.

Volume 2 details procedures for isolating and studying individual genes (preparation and screening of libraries, polymerase chain reactions, DNA sequencing and studying gene expression). It is assumed that the basics of Volume 1 are now in place, but the procedures are still described in the same down to earth fashion with protocols complemented by background information and troubleshooting hints.

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L'autore

Terry Brown is at UMIST, Manchester, UK.

Contenuti

  • 1: T.A. Brown: Strategies for research in molecular biology
  • 2: Anna-Maria Frischauf: Construction of genomic libraries in lambda and cosmic vectors
  • 3: Madan K. Bhattacharyya: Construction of a cDNA library
  • 4: C.R.Mundy, M. W.Cunningham and C.A.Read: Nucleic acid labelling and detection
  • 5: N.J.Dyson: Immobilization of nucleic acids and hybridization analysis
  • 6: T.A.Brown: DNA sequencing
  • 7: T.A.Brown: The polymerase chain reaction
  • 8: John M. Ward: Protein expression in Escherichia coli
  • 9: Colin P.Smith: Transcript mapping
  • Appendices1: Health hazards and safety procedures
  • 2: Equipment for molecular biology research
  • 3: Important Escherichia coli strains
  • 4: Recipes and general procedures
  • 5: Restriction endonucleases
  • 6: DNA and RNA modification enzymes
  • 7: List of suppliers' addresses

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