Protein Localization by Fluorescence Microscopy: A Practical Approach: 218 - Brossura

Brossura

ISBN 10: 0199637407 ISBN 13: 9780199637409

Casa editrice: OUP Oxford, 1999

Riassunto

There is an ever-increasing number of genes that have been sequenced but are of completely unknown function. The ability to determine the location of such gene products within the cell, either by the use of antibodies or by the production of chimeras with green fluorescent protein, is a vital step towards understanding what they do. This is one major reason why fluorescence microscopy is enjoying a revival. This no-nonsense guide provides detailed, practical advice on all aspects of the subject: from choosing the right equipment, to interpreting results. It balances the advantages of a wide range of techniques - including live cell work - against the potential pitfalls, offering invaluable "tricks of the trade" along the way. Protein Localization byFluorescence Light Microscopy: A Practical Approach has something to offer all microscopists, giving a solid grounding to the novice whilst extending the range of the experienced user.

Su questo libro

Recensione

... a useful bencjh-aid. The Editor-Victoria Allan - ans all of the contributors should be congratulated on putting together a valuable reference text (Trends Cell Biology)

Contenuti

• Preface
• 1: Allan: Basic Immunofluorescence
• 2: North and Small: Immunofluorescent Labelling of Sections
• 3: Ersfeld and Stone:
• 4: Amos: Instruments for Fluorescence Imaging
• 5: Pepperkok and Shima: Fluorescence Microscopy of Living Vertebrate Cells
• 6: Davis: Visualising Fluorescence in Drosophila - optimal detection in thick specimens
• 7: Hawes, Boevink and Moore: Green Fluorescent Protein in Plants
• 8: Hagan and Ayscough: Fluorescence Microscopy in Yeast
• Index

Dettagli dell'edizione

• Editore: OUP Oxford
• Data di pubblicazione: 1999
• Lingua: Inglese
• ISBN 10: 0199637407
• ISBN 13: 9780199637409
• Rilegatura: Copertina flessibile
• Numero di pagine: 256
• Redattore: Allan Viki
• Contatto del produttore: non disponibile
• Persona responsabile: Ufficio Anagrafiche Messaggerie; ufficio.anagrafiche@meli.it; ; Via Verdi, 8; Assago Milano; 20090; Italia